Surface-Independent Antibacterial Coating Using Silver Nanoparticle-Generating Engineered Mussel Glue

• Published in: ACS applied materials & interfaces Vol. 6; no. 22; pp. 20242 - 20253
• Main Authors: Jo, Yun Kee, Seo, Jeong Hyun, Choi, Bong-Hyuk, Kim, Bum Jin, Shin, Hwa Hui, Hwang, Byeong Hee, Cha, Hyung Joon
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 26.11.2014
• Subjects: Amino Acid Sequence; Animals; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Bivalvia - metabolism; Cell Line; Coated Materials, Biocompatible - chemistry; Coated Materials, Biocompatible - metabolism; Gram-Negative Bacteria - drug effects; Gram-Positive Bacteria - drug effects; Metal Nanoparticles - chemistry; Metal Nanoparticles - toxicity; Mice; Nanofibers - chemistry; Nanofibers - ultrastructure; Peptides - genetics; Peptides - metabolism; Polystyrenes - chemistry; Proteins - chemistry; Proteins - genetics; Proteins - metabolism; Recombinant Fusion Proteins - biosynthesis; Recombinant Fusion Proteins - chemistry; Recombinant Fusion Proteins - genetics; Silver Nitrate - chemistry; Surface Properties; silver binding peptide; antibacterial coating; silver nanoparticle; mussel adhesive protein; surface-independent coating
• ISSN: 1944-8244; 1944-8252
• DOI: 10.1021/am505784k

During implant surgeries, antibacterial agents are needed to prevent bacterial infections, which can cause the formation of biofilms between implanted materials and tissue. Mussel adhesive proteins (MAPs) derived from marine mussels are bioadhesives that show strong adhesion and coating ability on various surfaces even in wet environment. Here, we proposed a novel surface-independent antibacterial coating strategy based on the fusion of MAP to a silver-binding peptide, which can synthesize silver nanoparticles having broad antibacterial activity. This sticky recombinant fusion protein enabled the efficient coating on target surface and the easy generation of silver nanoparticles on the coated-surface under mild condition. The biosynthesized silver nanoparticles showed excellent antibacterial efficacy against both Gram-positive and Gram-negative bacteria and also revealed good cytocompatibility with mammalian cells. In this coating strategy, MAP-silver binding peptide fusion proteins provide hybrid environment incorporating inorganic silver nanoparticle and simultaneously mediate the interaction of silver nanoparticle with surroundings. Moreover, the silver nanoparticles were fully synthesized on various surfaces including metal, plastic, and glass by a simple, surface-independent coating manner, and they were also successfully synthesized on a nanofiber surface fabricated by electrospinning of the fusion protein. Thus, this facile surface-independent silver nanoparticle-generating antibacterial coating has great potential to be used for the prevention of bacterial infection in diverse biomedical fields.