Highly Water-Soluble BODIPY-Based Fluorescent Probe for Sensitive and Selective Detection of Nitric Oxide in Living Cells

A highly water-soluble BODIPY dye bearing electron-rich o-diaminophenyl groups at 2,6-positions was prepared as a highly sensitive and selective fluorescent probe for detection of nitric oxide (NO) in living cells. The fluorescent probe displays an extremely weak fluorescence with fluorescence quant...

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Published inACS applied materials & interfaces Vol. 5; no. 10; pp. 4107 - 4112
Main Authors K. Vegesna, Giri, Sripathi, Srinivas R, Zhang, Jingtuo, Zhu, Shilei, He, Weilue, Luo, Fen-Tair, Jahng, Wan Jin, Frost, Megan, Liu, Haiying
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 22.05.2013
Subjects
Animals
Boron Compounds - chemistry
Cell Line
Fluorescent Dyes - chemistry
Limit of Detection
Magnetic Resonance Spectroscopy
Mice
Nitric Oxide - analysis
Solubility
Water - chemistry
cell imaging
nitric oxide
fluorescent probe
BODIPY dyes
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Summary:A highly water-soluble BODIPY dye bearing electron-rich o-diaminophenyl groups at 2,6-positions was prepared as a highly sensitive and selective fluorescent probe for detection of nitric oxide (NO) in living cells. The fluorescent probe displays an extremely weak fluorescence with fluorescence quantum yield of 0.001 in 10 mM phosphate buffer (pH 7.0) in the absence of NO as two electron-rich o-diaminophenyl groups at 2,6-positions significantly quench the fluorescence of the BODIPY dye via photoinduced electron transfer mechanism. The presence of NO in cells enhances the dye fluorescence dramatically. The fluorescent probe demonstrates excellent water solubility, membrane permeability, and compatibility with living cells for sensitive detection of NO.
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ISSN:1944-8244
1944-8252
DOI:10.1021/am303247s