Conclusive Evidence of the Reconstituted Hexasome Proven by Native Mass Spectrometry

It has been suggested that the hexasome, in which one of the H2A/H2B dimers is depleted from the canonical nucleosome core particle (NCP), is an essential intermediate during NCP assembly and disassembly, but little structural evidence of this exists. In this study, reconstituted products in a conve...

Full description

Saved in:
Bibliographic Details
Published inBiochemistry (Easton) Vol. 52; no. 31; pp. 5155 - 5157
Main Authors Azegami, Nanako, Saikusa, Kazumi, Todokoro, Yasuto, Nagadoi, Aritaka, Kurumizaka, Hitoshi, Nishimura, Yoshifumi, Akashi, Satoko
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 06.08.2013
Subjects
Dimerization
Histones - chemistry
Histones - metabolism
Nucleosomes - chemistry
Nucleosomes - metabolism
Spectrometry, Mass, Electrospray Ionization
Online AccessGet full text

Cover

More Information
Summary:It has been suggested that the hexasome, in which one of the H2A/H2B dimers is depleted from the canonical nucleosome core particle (NCP), is an essential intermediate during NCP assembly and disassembly, but little structural evidence of this exists. In this study, reconstituted products in a conventional NCP preparation were analyzed by native electrospray ionization mass spectrometry, and it was found that the hexasome, which migrated in a manner almost identical to that of the octasome NCP in native polyacrylamide gel electrophoresis, was produced simultaneously with the octasome NCP. This result might contribute to understanding the assembly and disassembly mechanism of NCPs.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0006-2960
1520-4995
DOI:10.1021/bi4005655