Spontaneous Coassembly of Biologically Active Nanoparticles via Affinity Binding of Heparin-Binding Proteins to Alginate-Sulfate

• Published in: Nano letters Vol. 16; no. 2; pp. 883 - 888
• Main Authors: Ruvinov, Emil, Freeman, Inbar, Fredo, Roei, Cohen, Smadar
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 10.02.2016
• Subjects: Affinity; Alginates - chemistry; Animals; Binding; Biocompatible Materials - chemistry; Disease Models, Animal; Drug Carriers - administration & dosage; Drug Carriers - chemistry; Encapsulating; Glucuronic Acid - chemistry; Heparin - administration & dosage; Heparin - metabolism; Hexuronic Acids - chemistry; Hindlimb - pathology; Ischemia; Ischemia - drug therapy; Myocardial Infarction - drug therapy; Nanoparticles; Nanoparticles - administration & dosage; Nanoparticles - chemistry; Nanostructure; Protein Binding; Proteins; Regenerative Medicine; Repair; Sulfates - chemistry; repair; coassembly; nanoparticles; Affinity binding; heparin-binding proteins; alginate-sulfate
• ISSN: 1530-6984; 1530-6992
• DOI: 10.1021/acs.nanolett.5b03598

Controlled delivery of heparin-binding (HB) proteins represents a challenge in regenerative medicine strategies. Here, we describe the features of novel nanoparticles (NPs), spontaneously coassembled due to affinity interactions between HB proteins and the semisynthetic anionic polysaccharide, alginate-sulfate. The NPs efficiently encapsulated and protected the proteins from proteolysis. Injection of a combination of NPs encapsulating multiple therapeutic growth factors promoted effective and long-term tissue repair in animal models of severe ischemia (murine model of hindlimb ischemia and acute myocardial infarction in rats). This simple yet efficient NP fabrication method is amenable for clinical use.