Selective Detection of 5‑Formyl-2′-deoxycytidine in DNA Using a Fluorogenic Hydroxylamine Reagent

Fluorogenic hydroxylamine reagents were used for detecting 5-fC through a labeling pathway. Chemical synthesis, HPLC, denaturing PAGE, and DNA MS were applied to testify that the probe reacted with 5-fC with oligodeoxynucleotide selectivity to achieve 5-fC detection conveniently and quantificational...

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Bibliographic Details
Published inOrganic letters Vol. 15; no. 13; pp. 3266 - 3269
Main Authors Guo, Pu, Yan, Shengyong, Hu, Jianlin, Xing, Xiwen, Wang, Changcheng, Xu, Xiaowei, Qiu, Xiaoyu, Ma, Wen, Lu, Chunjiang, Weng, Xiaocheng, Zhou, Xiang
Format Journal Article
LanguageEnglish
Published WASHINGTON American Chemical Society 05.07.2013
Amer Chemical Soc
Subjects
Chemistry
Chemistry, Organic
Chromatography, High Pressure Liquid
Deoxycytidine - analogs & derivatives
Deoxycytidine - analysis
Deoxycytidine - chemistry
DNA - chemistry
Fluorescent Dyes - analysis
Fluorescent Dyes - chemistry
Hydroxylamine - chemistry
Indicators and Reagents - chemistry
Oligodeoxyribonucleotides - chemistry
Physical Sciences
Science & Technology
Spectrometry, Fluorescence
METHYLATION
5-METHYLCYTOSINE
CONVERSION
DEMETHYLATION
ISLANDS
5-HYDROXYMETHYLCYTOSINE
TET PROTEINS
EFFICIENT
5-FORMYLCYTOSINE
OLIGONUCLEOTIDES
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Summary:Fluorogenic hydroxylamine reagents were used for detecting 5-fC through a labeling pathway. Chemical synthesis, HPLC, denaturing PAGE, and DNA MS were applied to testify that the probe reacted with 5-fC with oligodeoxynucleotide selectivity to achieve 5-fC detection conveniently and quantificationally with the method of fluorescence. The feasibility of fluorescently detecting 5-fC in a genome was also investigated.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1523-7060
1523-7052
DOI:10.1021/ol401290d