Tritiated peptides. 12. Synthesis and biological activity of [4-3H-Phe8]substance P

Substance P has been prepared 3H labeled at Phe8 by catalytic deiodination of a protected precursor. Synthesis of the precursor was by solid-phase methodology on polydimethylacrylamide resin and by condensation in solution of fragments covering sequences 1-4, 5-7, and 8-11. Free peptide made by each...

Full description

Saved in:
Bibliographic Details
Published inJournal of medicinal chemistry Vol. 25; no. 10; pp. 1209 - 1213
Main Authors Allen, Mark C, Brundish, Derek E, Wade, Roy, Sandberg, Bengt E. B, Hanley, Michael R, Iversen, Leslie L
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 01.10.1982
Subjects
Amino Acids - analysis
Animals
Chemical Phenomena
Chemistry
Chromatography, High Pressure Liquid
Cross Reactions
Guinea Pigs
Ileum - metabolism
In Vitro Techniques
Isotope Labeling
Muscle Contraction - drug effects
Muscle, Smooth - drug effects
Substance P - immunology
Substance P - pharmacology
Tritium
Online AccessGet full text

Cover

More Information
Summary:Substance P has been prepared 3H labeled at Phe8 by catalytic deiodination of a protected precursor. Synthesis of the precursor was by solid-phase methodology on polydimethylacrylamide resin and by condensation in solution of fragments covering sequences 1-4, 5-7, and 8-11. Free peptide made by each route analyzed satisfactorily and had the same chromatographic characteristics as unlabeled substance P. It was indistinguishable from the latter by radioimmunoassay when N and C terminally directed antisera were used and in the ability to cause contractions of isolated guinea pig ileum. Specific radioactivity was 23 Ci/mmol.
Bibliography:istex:0E10957651F8F28320ABB8CAA02E35E3D40530F7
ark:/67375/TPS-2H0KCX2P-G
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0022-2623
1520-4804
DOI:10.1021/jm00352a022