DNA Origami Nanopores for Controlling DNA Translocation

We combine DNA origami structures with glass nanocapillaries to reversibly form hybrid DNA origami nanopores. Trapping of the DNA origami onto the nanocapillary is proven by imaging fluorescently labeled DNA origami structures and simultaneous ionic current measurements of the trapping events. We th...

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Bibliographic Details
Published inACS nano Vol. 7; no. 7; pp. 6024 - 6030
Main Authors Hernández-Ainsa, Silvia, Bell, Nicholas A. W, Thacker, Vivek V, Göpfrich, Kerstin, Misiunas, Karolis, Fuentes-Perez, Maria Eugenia, Moreno-Herrero, Fernando, Keyser, Ulrich F
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 23.07.2013
Subjects
Base Sequence
Binding sites
Capillary Action
Deoxyribonucleic acid
DNA - chemistry
DNA - genetics
DNA - isolation & purification
Folding
Glass
Materials Testing
Micromanipulation - methods
Molecular Sequence Data
Nanopores - ultrastructure
Nanostructure
Porosity
Sequence Analysis, DNA - methods
Trapping
Tuning
Versatility
nanotechnology
nanopores
biosensors
fluorescent imaging
DNA structures
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Summary:We combine DNA origami structures with glass nanocapillaries to reversibly form hybrid DNA origami nanopores. Trapping of the DNA origami onto the nanocapillary is proven by imaging fluorescently labeled DNA origami structures and simultaneous ionic current measurements of the trapping events. We then show two applications highlighting the versatility of these DNA origami nanopores. First, by tuning the pore size we can control the folding of dsDNA molecules (“physical control”). Second, we show that the specific introduction of binding sites in the DNA origami nanopore allows selective detection of ssDNA as a function of the DNA sequence (“chemical control”).
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ISSN:1936-0851
1936-086X
DOI:10.1021/nn401759r