Zinc Porphyrin as a Donor for FRET in Zn(II)cytochrome c

We demonstrate that Zn(II) porphyrin in Zn(II)cytochrome c (Zn cyt c) is a fluorescence resonance energy transfer (FRET) donor to an Alexa660 dye acceptor. The energy transfer efficiency is dependent on the distance between the two fluorophores as shown through protein denaturation studies of five Z...

Full description

Saved in:
Bibliographic Details
Published inJournal of the American Chemical Society Vol. 132; no. 6; pp. 1752 - 1753
Main Authors Lee, Andrea J, Ensign, Amy A, Krauss, Todd D, Bren, Kara L
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 17.02.2010
Subjects
Crystallography, X-Ray
Cytochromes c - chemistry
Fluorescence Resonance Energy Transfer
Fluorescent Dyes - chemistry
Metalloporphyrins - chemistry
Models, Molecular
Protein Conformation
Protein Folding
Zinc
Online AccessGet full text

Cover

More Information
Summary:We demonstrate that Zn(II) porphyrin in Zn(II)cytochrome c (Zn cyt c) is a fluorescence resonance energy transfer (FRET) donor to an Alexa660 dye acceptor. The energy transfer efficiency is dependent on the distance between the two fluorophores as shown through protein denaturation studies of five Zn cyt c variants labeled with Alexa660 in different positions. The relative quantum yield, excitation and emission energies, and labeling efficiencies of this donor−acceptor pair allow for a method of analysis based on sensitized emission of the acceptor. These studies show that Zn(II) porphyrin is an effective energy donor for measurement of molecular-scale distances by FRET.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0002-7863
1520-5126
DOI:10.1021/ja909106p