Molecular Structure of a Fibrillar Alzheimer's Aβ Fragment
Amyloid-β (Aβ) peptide deposition as fibrillar senile plaques is a key element in the pathology of Alzheimer's disease. Here we present a high-resolution structure of an Aβ amyloid fibril using magnetically aligned preparations of a central Aβ domain which forms representative amyloid fibrils....
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Published in | Biochemistry (Easton) Vol. 39; no. 43; pp. 13269 - 13275 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
American Chemical Society
31.10.2000
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Subjects | |
Online Access | Get full text |
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Summary: | Amyloid-β (Aβ) peptide deposition as fibrillar senile plaques is a key element in the pathology of Alzheimer's disease. Here we present a high-resolution structure of an Aβ amyloid fibril using magnetically aligned preparations of a central Aβ domain which forms representative amyloid fibrils. Diffraction analysis of these samples revealed Bragg reflections on layer lines consistent with a preferred orientation, as opposed to the typical symmetry associated with fibers. These crystalline properties permitted a molecular replacement approach based upon a β-hairpin motif resulting in a structure of the fibrillar Aβ peptide. This detailed molecular structure of Aβ in its fibrous state provides clues as to the mechanism of amyloid assembly and identifies potential targets for controlling the aggregation process. |
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Bibliography: | L.C.S. is funded by the Medical Research Council, U.K. P.E.F. acknowledges the support of the Ontario Mental Health Foundation, Alzheimer Association of Ontario, Scottish Rite Charitable Foundation and the Medical Research Council of Canada. ark:/67375/TPS-XG0FS506-7 istex:6BE88302DE766660832848AE7DA77F256776EEE7 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0006-2960 1520-4995 |
DOI: | 10.1021/bi000637v |