A Two-Photon Fluorescent Probe for Thiols in Live Cells and Tissues

We report a two-photon fluorescent probe (ASS) that can be excited by 780 nm femtosecond pulses and detect thiols in live cells and living tissues at a 90−180 μm depth without interference from other biologically relevant species by two-photon microscopy.

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Bibliographic Details
Published inJournal of the American Chemical Society Vol. 132; no. 4; pp. 1216 - 1217
Main Authors Lee, Jun Han, Lim, Chang Su, Tian, Yu Shun, Han, Ji Hee, Cho, Bong Rae
Format Journal Article
LanguageEnglish
Published WASHINGTON American Chemical Society 03.02.2010
Amer Chemical Soc
Subjects
Animals
Brain Chemistry
Chemistry
Chemistry, Multidisciplinary
Fluorescent Dyes - chemistry
HeLa Cells
Humans
Methylamines - chemistry
Microscopy, Fluorescence
Molecular Structure
Naphthalenes - chemistry
Photons
Physical Sciences
Rats
Science & Technology
Sulfhydryl Compounds - analysis
ACIDIC VESICLES
HOMOCYSTEINE
IONS
GLUTATHIONE
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Summary:We report a two-photon fluorescent probe (ASS) that can be excited by 780 nm femtosecond pulses and detect thiols in live cells and living tissues at a 90−180 μm depth without interference from other biologically relevant species by two-photon microscopy.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0002-7863
1520-5126
DOI:10.1021/ja9090676