Mass Spectral Characterization of Lipooligosaccharides from Haemophilus influenzae 2019
Lipooligosaccharide (LOS) glycoforms from Haemophilus influenzae 2019 were profiled using the high-resolution and accurate mass capabilities of Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry. Sequence and linkage for two previously unknown LOS glycoforms were subsequently obtai...
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Published in | Biochemistry (Easton) Vol. 39; no. 40; pp. 12406 - 12414 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
United States
American Chemical Society
10.10.2000
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Subjects | |
Online Access | Get full text |
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Summary: | Lipooligosaccharide (LOS) glycoforms from Haemophilus influenzae 2019 were profiled using the high-resolution and accurate mass capabilities of Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry. Sequence and linkage for two previously unknown LOS glycoforms were subsequently obtained through MS n analyses on FT-ICR and quadrupole ion trap (qIT) instruments. MS n analysis of negative ion precursors confirmed structural details within the lipid moiety, while CID spectra of sodiated precursor ions provided monosaccharide sequence and linkage for the oligosaccharide portion of the molecule. Results obtained in this study indicate that extensive heterogeneity exists within the oligosaccharide moieties in LOS from H. influenzae 2019. More importantly, the data suggest that additional hexose moieties, which are added onto the LOS, are not simple extensions of one particular core structure but rather that structural isomers with different connectivities are present within the heterogeneous mixture. |
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Bibliography: | istex:5FD290BAC7A7AF12BA14DC3A46D4120EED939BCD ark:/67375/TPS-8XG0NKGW-W Funding for this research was provided by National Institutes of Health Grants GM47356 (to J.A.L.) and AI31254 and AI24616 (to B.W.G.). ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0006-2960 1520-4995 |
DOI: | 10.1021/bi001181k |