A Comprehensive Analysis of the Soybean Genes and Proteins Expressed under Flooding Stress using Transcriptome and Proteome Techniques

• Published in: Journal of proteome research Vol. 8; no. 10; pp. 4766 - 4778
• Main Authors: Komatsu, Setsuko, Yamamoto, Ryo, Nanjo, Yohei, Mikami, Yoji, Yunokawa, Harunobu, Sakata, Katsumi
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 01.10.2009
• Subjects: Alcohols - metabolism; Cysteine Proteinase Inhibitors; Electrophoresis, Gel, Two-Dimensional; Ethylenes - metabolism; Floods; Gene Expression Profiling - methods; Gene Expression Regulation, Plant; Gene Regulatory Networks; Genes, Plant; Glucosyltransferases - metabolism; Glycine max - genetics; Glycine max - metabolism; Heat-Shock Proteins - metabolism; Hypocotyl - metabolism; Molecular Chaperones - metabolism; Plant Proteins - classification; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Roots - metabolism; Proteome - metabolism; Proteomics - methods; Reactive Oxygen Species - metabolism; Stress, Physiological; Time Factors; soybean; flooding stress; transcriptome; proteome
• ISSN: 1535-3893; 1535-3907
• DOI: 10.1021/pr900460x

The inducible genes and proteins were analyzed using transcriptome and proteome techniques to explore the mechanisms underlying soybean response to flooding stress. Soybean seedlings were germinated for 2 days and subjected to flooding for 12 h, and the total RNAs and proteins were extracted from the root and hypocotyl. High-coverage gene expression profiling analysis as transcriptome technique was performed. Ninety-seven out of the 29 388 peaks observed demonstrated a greater than 25-fold change following 12 h of flood-induced stress. Furthermore, 34 proteins out of 799 proteins were changed by 12 h stress. Genes associated with alcohol fermentation, ethylene biosynthesis, pathogen defense, and cell wall loosening were significantly up-regulated. Hemoglobin, acid phosphatase, and Kunitz trypsin protease inhibitor were altered at both transcriptional and translational levels. Reactive oxygen species scavengers and chaperons were changed only at the translational level. It is suggested that the early response of soybean under flooding might be important stress adaptation to ensure survival against not only hypoxia but also the direct damage of cell by water.