Structural Biology of Viruses by the Combination of Electron Cryomicroscopy and X-ray Crystallography

Recent developments in electron cryomicroscopy and image analysis have made it a powerful tool to investigate the structure, assembly, and dynamics of biological supramolecular assemblies. The subjects of study now include a variety of biological samples that may be homogeneous or heterogeneous, sym...

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Bibliographic Details
Published inBiochemistry (Easton) Vol. 41; no. 39; pp. 11517 - 11524
Main Authors Tang, Liang, Johnson, John E
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 01.10.2002
Subjects
Cryoelectron Microscopy - methods
Crystallography, X-Ray - methods
Ribosomes - chemistry
Ribosomes - ultrastructure
Ribosomes - virology
Structure-Activity Relationship
Viral Proteins - chemistry
Viral Proteins - ultrastructure
Viruses - chemistry
Viruses - ultrastructure
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Summary:Recent developments in electron cryomicroscopy and image analysis have made it a powerful tool to investigate the structure, assembly, and dynamics of biological supramolecular assemblies. The subjects of study now include a variety of biological samples that may be homogeneous or heterogeneous, symmetric or nonsymmetric. The combination of this technique with X-ray crystallography plays an increasingly important role in structural biology and provides unique structural information for understanding large, complex biological systems. Here we provide an overview of the technologies and specific applications to virus structure and function.
Bibliography:ark:/67375/TPS-2TZK727Q-L
We acknowledge grants from the National Institutes of Health (GM34220, GM54076, and AI40101) for support of the authors and work from our laboratory presented in this review.
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ISSN:0006-2960
1520-4995
DOI:10.1021/bi020170j