Highly Sequence-Dependent Formation of Fluorescent Silver Nanoclusters in Hybridized DNA Duplexes for Single Nucleotide Mutation Identification

Fluorescent silver nanoclusters were successfully synthesized using hybridized DNA duplexes as capping scaffolds. The formation of these emitters was highly sequence-dependent and could specifically identify a single nucleotide mutation, the sickle cell anemia gene mutation. Furthermore, the identif...

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Bibliographic Details
Published inJournal of the American Chemical Society Vol. 132; no. 3; pp. 932 - 934
Main Authors Guo, Weiwei, Yuan, Jipei, Dong, Qingzhe, Wang, Erkang
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 27.01.2010
Subjects
Anemia, Sickle Cell - genetics
Base Sequence
DNA - chemistry
DNA - genetics
Fluorescence
Humans
Metal Nanoparticles - chemistry
Nucleic Acid Hybridization
Point Mutation - genetics
Polymorphism, Single Nucleotide - genetics
Sequence Analysis, DNA
Silver - chemistry
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Summary:Fluorescent silver nanoclusters were successfully synthesized using hybridized DNA duplexes as capping scaffolds. The formation of these emitters was highly sequence-dependent and could specifically identify a single nucleotide mutation, the sickle cell anemia gene mutation. Furthermore, the identification of single-nucleotide differences using this strategy was extended to more general types of single-nucleotide mismatches.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0002-7863
1520-5126
DOI:10.1021/ja907075s