Sulfur Oxygenates of Biomimetics of the Diiron Subsite of the [FeFe]-Hydrogenase Active Site: Properties and Oxygen Damage Repair Possibilities

• Published in: Journal of the American Chemical Society Vol. 131; no. 23; pp. 8296 - 8307
• Main Authors: Liu, Tianbiao, Li, Bin, Singleton, Michael L, Hall, Michael B, Darensbourg, Marcetta Y
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 17.06.2009
• Subjects: Biomimetic Materials - chemistry; Catalytic Domain; Hydrogenase - chemistry; Iron - chemistry; Iron-Sulfur Proteins - chemistry; Models, Molecular; Oxygen - chemistry; Sulfur - chemistry
• ISSN: 0002-7863; 1520-5126
• DOI: 10.1021/ja9016528

This study explores the site specificity (sulfur vs the Fe−Fe bond) of oxygenation of diiron (FeIFeI and FeIIFeII) organometallics that model the 2-iron subsite in the active site of [FeFe]-hydrogenase: (μ-pdt)[Fe(CO)2L][Fe(CO)2L′] (L = L′ = CO (1); L = PPh3, L′ = CO (2); L = L′ = PMe3 (4)) and (μ-pdt)(μ-H)[Fe(CO)2PMe3]2 (5). DFT computations find that the Fe−Fe bond in the FeIFeI diiron models is thermodynamically favored to produce the μ-oxo or oxidative addition product, FeII−O−FeII; nevertheless, the sulfur-based HOMO−1 accounts for the experimentally observed mono- and bis-O-atom adducts at sulfur, i.e., (μ-pst)[Fe(CO)2L][Fe(CO)2L′] (pst = −S(CH2)3S(O)−, 1,3-propanesulfenatothiolate; L = L′ = CO (1-O); L = PPh3, L′ = CO (2-O); L = L′ = PMe3 (4-O)) and (μ-pds)[Fe(CO)2L][Fe(CO)2L′] (pds = −(O)S(CH2)3S(O)−, 1,3-propanedisulfenato; L = PPh3, L′ = CO (2-O 2 )). The FeII(μ-H)FeII diiron model (5), for which the HOMO is largely of sulfur character, exclusively yields S-oxygenation. The depressing effect of such bridging ligand modification on the dynamic NMR properties arising from rotation of the Fe(CO)3 correlates with higher barriers to the CO/PMe3 exchange of (μ-pst)[Fe(CO)3]2 as compared to (μ-pdt)[Fe(CO)3]2. Five molecular structures are confirmed by X-ray diffraction: 1-O, 2-O, 2-O 2 , 4-O, and 6. Deoxygenation with reclamation of the μ-pdt parent complex occurs in a proton/electron-coupled process. The possible biological relevance of oxygenation and deoxygenation studies is discussed.