2,2‘-Diphenyl-1-picrylhydrazyl Radical-Scavenging Active Components from Adlay (Coix lachryma-jobi L. Var. ma-yuen Stapf) Hulls

• Published in: Journal of agricultural and food chemistry Vol. 50; no. 21; pp. 5850 - 5855
• Main Authors: Kuo, Ching-Chuan, Chiang, Wenchang, Liu, Guey-Ping, Chien, Ya-Lin, Chang, Jang-Yang, Lee, Ching-Kuo, Lo, Jir-Mehng, Huang, Shou-Ling, Shih, Ming-Chih, Kuo, Yueh-Hsiung
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 09.10.2002
• Subjects: Agronomy. Soil science and plant productions; Antioxidants - analysis; Antioxidants - pharmacology; Biological and medical sciences; Biphenyl Compounds; Chemical constitution; Chromatography, High Pressure Liquid; Economic plant physiology; Free Radical Scavengers - pharmacology; Fundamental and applied biological sciences. Psychology; General pharmacology; Magnetic Resonance Spectroscopy; Medical sciences; Methanol; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Picrates; Plant Extracts - chemistry; Plant Structures - chemistry; Poaceae - chemistry; Seeds - chemistry; Cereal bran; Plant tissue; Monocotyledones; Fractionation; Purification; Pharmacognosy; Chemical structure; Adlay hulls; Identification; Antioxidant; Radical scavenger; antioxidant activity; Cereal crop; Biological activity; Tissue extract; Gramineae; Angiospermae; Spermatophyta; free radical; Coix lacryma jobi; Comparative study; Methanol; active components
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf020391w

An activity-directed fractionation and purification process was used to identify the antioxidative components of adlay hulls. Hulls of adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) were extracted with methanol and then separated into water, 1-butanol, ethyl acetate, and hexane fractions. The 1-butanol-soluble fraction exhibited greater capacity to scavenge 2,2‘-diphenyl-1-picrylhydrazyl (DPPH) radicals when compared with fractions soluble in water, ethyl acetate, and hexane phases. The 1-butanol fraction was then subjected to separation and purification using Diaion HP-20 chromatography, silica gel chromatography, and HPLC. Six compounds showing strong antioxidant activity were identified by spectroscopic methods (1H NMR, 13C NMR, IR, and MS) and by comparison with authentic samples to be coniferyl alcohol (1), syringic acid (2), ferulic acid (3), syringaresinol (4), 4-ketopinoresinol (5), and a new lignan, mayuenolide (6). Keywords: Adlay hulls; free radical; antioxidant activity; active components