Quencher-Free, End-Stacking Oligonucleotides for Probing Single-Base Mismatches in DNA

Fluorescence can be quenched through PET from the fluorophore to neighboring C, T, and G bases, but not to the A moiety. The hairpin stem stability arising from π-stacking and the PET between the pyrene-labeled 2‘-deoxynucleotide units and their neighboring bases are the two main factors that affect...

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Bibliographic Details
Published inOrganic letters Vol. 7; no. 22; pp. 4931 - 4933
Main Authors Seo, Young Jun, Ryu, Jin Ho, Kim, Byeang Hyean
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 27.10.2005
Subjects
Base Pair Mismatch
Base Sequence
DNA - analysis
DNA - chemistry
Fluorescence Resonance Energy Transfer
Fluorescent Dyes - chemical synthesis
Fluorescent Dyes - chemistry
Molecular Sequence Data
Oligonucleotide Probes - chemical synthesis
Oligonucleotide Probes - chemistry
Pyrenes - chemistry
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Summary:Fluorescence can be quenched through PET from the fluorophore to neighboring C, T, and G bases, but not to the A moiety. The hairpin stem stability arising from π-stacking and the PET between the pyrene-labeled 2‘-deoxynucleotide units and their neighboring bases are the two main factors that affect the operation of these novel fluorescent oligonucleotides.
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ISSN:1523-7060
1523-7052
DOI:10.1021/ol0518582