Preparation, Physical Properties, On-Bead Binding Assay and Spectroscopic Reliability of 25 Barcoded Polystyrene−Poly(ethylene glycol) Graft Copolymers

• Published in: Journal of the American Chemical Society Vol. 125; no. 35; pp. 10546 - 10560
• Main Authors: Fenniri, Hicham, Chun, Sangki, Ding, Lunhan, Zyrianov, Yegor, Hallenga, Klaas
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 03.09.2003
• Subjects: Applied sciences; Exact sciences and technology; Organic polymers; Physicochemistry of polymers; Properties and characterization; Special properties (catalyst, reagent or carrier); Alkaline phosphatase; Graft copolymer; Phosphoric monoester hydrolases; Combinatorial chemistry; Molecular interaction; Esterases; NMR spectrometry; Styrene derivative copolymer; Grafting; Characterization; Hydrogen 1; Styrene derivatives; Raman spectrometry; Chemical properties; Reaction support; Suspension polymerization; Carbon 13; Differential scanning calorimetry; Swelling; Enzyme; Ethylene oxide copolymer; Physical properties; Latex; Infrared spectrometry; Preparation; Hydrolases; Property structure relationship; Peptide synthesis; Spectrophotometry
• ISSN: 0002-7863; 1520-5126
• DOI: 10.1021/ja035665q

Here we describe the preparation of 25 beaded polystyrene−poly(ethylene glycol) graft copolymers from six spectroscopically active styrene monomers:  styrene, 2,5-dimethylstyrene, 4-methylstyrene, 2,4-dimethylstyrene, 4-tert-butylstyrene, and 3-methylstyrene. These polymers were thoroughly characterized by Raman, infrared, and 1H/13C NMR spectroscopies, and differential scanning calorimetry. Determination of the swelling properties, peptide synthesis, and on-bead streptavidin−alkaline phosphatase (SAP) binding assay further established that their physical and chemical properties where not significantly altered by the diversity of their encoded polystyrene core. Each of the 25 resins displayed a unique Raman and infrared vibrational fingerprint, which was converted into a “spectroscopic barcode”. The position of each bar matches the peak wavenumber in the corresponding spectrum but is independent of its intensity. From this simplified representation similarity maps comparing 35 000 resin pairs were generated to establish the spectroscopic barcoding as a reliable encoding methodology. In effect, in 99% of the cases, the highest similarity coefficients were obtained for resin pairs prepared from the same styrene derivatives even after SAP binding assay. We have also shown that a small but unique combination of a resin's vibrations (30−40%) is sufficient for its identification. However, in rare cases where a resin's vibrational signature has been severely compromised, both the Raman and infrared barcodes were synergistically and reliably utilized to unequivocally identify its chemical make up.