Determination of Random- and Blockwise-type De-esterified Pectins by Capillary Zone Electrophoresis

• Published in: Journal of agricultural and food chemistry Vol. 49; no. 11; pp. 5584 - 5588
• Main Authors: Jiang, Chii-Ming, Wu, Ming-Chang, Chang, Wei-Hsien, Chang, Hung-Min
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.11.2001
• Subjects: Biological and medical sciences; Electrophoresis, Capillary - methods; Esterification; Food industries; Fundamental and applied biological sciences. Psychology; General aspects; Methods of analysis, processing and quality control, regulation, standards; Pectins - analysis; Pectins - chemistry; Zone electrophoresis; Basic solution; Enzyme; Capillary electrophoresis; Hydrolases; Esterases; Application method; Pectin; Measurement method; Esterification; Pectinesterase; Carboxylic ester hydrolases
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf0105642

Capillary zone electrophoresis (CZE) was employed to determine the correlations between migration time and degree of esterification (DE) of pectinesterase-de-esterified pectins (PDPs) and alkaline-de-esterified pectins (ADPs) using 50 mM phosphate buffer (pH 6.5) as carrier electrolyte solution and 15 KV as applied voltage. Results showed that pectins with higher DEs exhibited shorter migration times. Linear correlation (r = 0.995) between migration time and DE of ADPs was observed, whereas down-curve correlation in PDPs was observed, regardless of the capillary length used (effective length, 30 and 60 cm). In addition, PDP appeared to migrate faster than ADP with the same DE under the same experimental conditions. Keywords: Degree of esterification; random-type de-esterified pectin; blockwise-type de-esterified pectin; capillary zone electrophoresis