Attomolar Protein Detection Using in-Hole Surface Plasmon Resonance

An in-hole nanohole surface plasmon resonance sensing scheme is demonstrated. Arrays of periodic nanoholes milled through thin layers of SiO x and gold were used to detect the binding of organic and biological molecules inside the nanoholes, while blocking the gold surfaces outside the holes. This n...

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Published inJournal of the American Chemical Society Vol. 131; no. 2; pp. 436 - 437
Main Authors Ferreira, Jacqueline, Santos, Marcos J. L, Rahman, Mohammad M, Brolo, Alexandre G, Gordon, Reuven, Sinton, David, Girotto, Emerson M
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 21.01.2009
Subjects
Biotin - chemistry
Cysteamine - chemistry
Gold - chemistry
Metal Nanoparticles - chemistry
Oxides - chemistry
Proteins - analysis
Proteins - chemistry
Silicon Compounds - chemistry
Streptavidin - chemistry
Surface Plasmon Resonance - methods
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Summary:An in-hole nanohole surface plasmon resonance sensing scheme is demonstrated. Arrays of periodic nanoholes milled through thin layers of SiO x and gold were used to detect the binding of organic and biological molecules inside the nanoholes, while blocking the gold surfaces outside the holes. This new approach is more efficient than the previous nanohole array method, where the response was related to binding events taking place inside of the holes and on the top gold surface. The improved sensitivity to binding events and lower detection limit are related to resonant surface plasmon enhanced transmission through the arrays of nanoholes. The sensitivity was found to be 650nm/RIU and the detection of three attomoles of proteins was estimated from this scheme.
Bibliography:ObjectType-Article-1
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ISSN:0002-7863
1520-5126
DOI:10.1021/ja807704v