Callipeltoside A: Total Synthesis, Assignment of the Absolute and Relative Configuration, and Evaluation of Synthetic Analogues

• Published in: Journal of the American Chemical Society Vol. 124; no. 35; pp. 10396 - 10415
• Main Authors: Trost, Barry M, Gunzner, Janet L, Dirat, Olivier, Rhee, Young H
• Format: Journal Article
• Language: English
• Published: WASHINGTON American Chemical Society 04.09.2002; Amer Chemical Soc
• Subjects: Anti-Bacterial Agents - chemical synthesis; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antibiotics, Antineoplastic - chemical synthesis; Antibiotics, Antineoplastic - chemistry; Antibiotics, Antineoplastic - pharmacology; Antineoplastic agents; Biological and medical sciences; Chemistry; Chemistry, Multidisciplinary; Exact sciences and technology; Female; General aspects; Heterocyclic compounds; Heterocyclic compounds with o, s, se, te hetero atom and condensed derivatives; Humans; Macrolides; Medical sciences; Molecular Conformation; Organic chemistry; Ovarian Neoplasms - drug therapy; Pharmacology. Drug treatments; Physical Sciences; Preparations and properties; Science & Technology; Stereoisomerism; Tumor Cells, Cultured; GLYCOSIDES; ASYMMETRIC-SYNTHESIS; DIELS-ALDER REACTIONS; CONVERSION; CONVENIENT METHOD; PALLADIUM-CATALYZED DECARBONYLATION; TRISUBSTITUTED ALKENES; MACROLIDE; CARBONYL-COMPOUNDS; ENANTIOSELECTIVE TOTAL-SYNTHESIS; Antineoplastic agent; Molecular structure; Aglycone; Absolute configuration; Oxygen heterocycle; Total synthesis; Macrocycle
• ISSN: 0002-7863; 1520-5126
• DOI: 10.1021/ja0205232

The total synthesis of the novel antitumor agent callipeltoside A, as well as several analogues, is accomplished and allows assignment of the stereochemistry not previously established. A convergent strategy is employed wherein the target is dissected into three unitsthe core macrolactone, the sugar callipeltose, and a cyclopropyl bearing chain. The strategy for the synthesis of the macrolactone derives from employment of diastereoselective aldol reactions that emanate from an 11 carbon piece. The stereochemistry of the latter derives from the chiral pool and two asymmetric reactionsa ketone reduction using CBS−oxazaborolidine and a Pd catalyzed asymmetric allylic alkylation (AAA). The novelty of the latter protocol is its control of regioselectivity as well as absolute configuration. The trisubstituted olefin is generated using an alkene−alkyne coupling to create a trisubustituted olefin with complete control of geometry. The excellent chemo- and regioselectivity highlights the synthetic potential of this new ruthenium catalyzed process. The macrolactonization employs in situ formation of an acylketene generated by the thermolysis of a m-dioxolenone. Two strategies evolved for attachment of the side chainone based upon olefination and a second upon olefin metathesis. The higher efficiency of the latter makes it the method of choice. A novel one pot olefin metathesis−Takai olefination protocol that should be broadly applicable is developed. The sugar is attached by a glycosylation by employing the O-trichloroacetimidate. This route provided both C-13 epimers of the macrolactone by using either enantiomeric ligand in the Pd AAA reaction. It also provided both trans-chlorocyclopropane diastereomers of callipeltoside A which allows the C-20 and C-21 configuration to be established as S and R, respectively. The convergent nature of the synthesis in which the largest piece, the macrolatone, require only 16 linear steps imparts utility to this strategy for the establishment of the structure−activity relationship. Initial biological testing demonstrates the irrelevance of the chloro substituent and the necessity of the sugar.