Fabrication of Polyion Complex Vesicles with Enhanced Salt and Temperature Resistance and Their Potential Applications as Enzymatic Nanoreactors

• Published in: Biomacromolecules Vol. 15; no. 7; pp. 2389 - 2397
• Main Authors: Chuanoi, Sayan, Anraku, Yasutaka, Hori, Mao, Kishimura, Akihiro, Kataoka, Kazunori
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 14.07.2014
• Subjects: Applied sciences; Chemical Phenomena; Complement C8 - chemistry; Enzymes - chemistry; Exact sciences and technology; Forms of application and semi-finished materials; Ions - chemistry; Magnetic Resonance Spectroscopy; Microscopy, Electron, Transmission; Miscellaneous; Nanoparticles - chemistry; Nanotechnology; Polymer industry, paints, wood; Polymers - chemistry; Sodium Chloride - chemistry; Technology of polymers; Temperature; Mixing; Salt resistance; Aminoacid copolymer; Thermal stability; Enzymatic activity; Interpolymer; Microencapsulation; Chemical properties; Acid copolymer; Manufacturing; Growth from solution; β-Galactosidase; Vesicle; Enzyme; Aspartic derivative copolymer; Ethylene oxide copolymer; Experimental study; Dimension spectrum; Aminoacid polymer; Glycosylases; Polyelectrolyte; Amine polymer; Glycosidases; Diblock copolymer; Hydrolases; Aspartic derivative polymer
• ISSN: 1525-7797; 1526-4602
• DOI: 10.1021/bm500127g

Integrating catalytic functions into polymeric vesicles through enzyme entrapment is appealing for bioreactor fabrication, yet there are critical issues regarding the regulation of solute transport through membranes and enzyme loading without denaturation. Polyion complex vesicles (PICsomes) with semipermeable membranes and the propensity to form in water can overcome these issues; however, cross-linking is required for sufficient physiological stability. Herein, we report the first successful fabrication of non-cross-linked PICsomes with sufficient stability at physiological salinity and temperature by tuning the hydrophobicity of the aliphatic side chains in the pendant group of the constituent polyelectrolytes. Dynamic light scattering and transmission electron microscopy revealed that the intervesicular fusion and disintegration of the PICsomes was prevented and a narrow distribution was maintained at physiological salinity and temperatures. Furthermore, their application as enzymatic nanoreactors was verified even in the presence of proteases. As such, the potential utility of the PICsomes in biomedical fields was established.