New ACE-Inhibitory Peptides from Hemp Seed (Cannabis sativa L.) Proteins

• Published in: Journal of agricultural and food chemistry Vol. 65; no. 48; pp. 10482 - 10488
• Main Authors: Orio, Lara P., Boschin, Giovanna, Recca, Teresa, Morelli, Carlo F., Ragona, Laura, Francescato, Pierangelo, Arnoldi, Anna, Speranza, Giovanna
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 06.12.2017
• Subjects: Angiotensin-Converting Enzyme Inhibitors - chemistry; Angiotensin-Converting Enzyme Inhibitors - isolation & purification; Cannabis - chemistry; Chromatography, High Pressure Liquid; Hydrolysis; Mass Spectrometry; Peptides - chemistry; Peptides - isolation & purification; Peptidyl-Dipeptidase A - chemistry; Plant Proteins - chemistry; Protein Hydrolysates - chemistry; Seeds - chemistry; bioactive peptides; LC-MS/MS; hemp seed protein hydrolysate; Cannabis sativa L; ACE-inhibitors
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/acs.jafc.7b04522

A hemp seed protein isolate, prepared from defatted hemp seed meals by alkaline solubilization/acid precipitation, was subjected to extensive chemical hydrolysis under acid conditions (6 M HCl). The resulting hydrolysate was fractionated by semipreparative RP-HPLC, and the purified fractions were tested as inhibitors of angiotensin converting enzyme (ACE). Mono- and bidimensional NMR experiments and LC-MS analyses led to the identification of four potentially bioactive peptides, i.e. GVLY, IEE, LGV, and RVR. They were prepared by solid-phase synthesis, and tested for ACE-inhibitory activity. The IC50 values were GVLY 16 ± 1.5 μM, LGV 145 ± 13 μM, and RVR 526 ± 33 μM, confirming that hemp seed may be a valuable source of hypotensive peptides.