Fecal and Oral Shedding of Helicobacter pylori From Healthy Infected Adults

• Published in: JAMA : the journal of the American Medical Association Vol. 282; no. 23; pp. 2240 - 2245
• Main Authors: Parsonnet, Julie, Shmuely, Haim, Haggerty, Thomas
• Format: Journal Article
• Language: English
• Published: Chicago, IL American Medical Association 15.12.1999
• Subjects: Adult; Air Microbiology; Bacteria; Biological and medical sciences; Carrier State - transmission; DNA Fingerprinting; Feces; Feces - virology; Gastrointestinal Contents - virology; Helicobacter Infections - transmission; Helicobacter pylori; Helicobacter pylori - genetics; Helicobacter pylori - isolation & purification; Helicobacter pylori - physiology; Humans; Investigative techniques, diagnostic techniques (general aspects); Medical sciences; Miscellaneous. Technology; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Polymerase Chain Reaction; Public health; RNA, Ribosomal, 16S - genetics; Saliva - virology; USA, California, Northern; Virus Shedding; Vomiting; Human; Evaluation; Spirillales; Oral administration; Spirillaceae; Biology; Asymptomatic; Experimental study; Bacteriological investigation; Infection; Helicobacter pylori; Bacteriosis; Bacteria; Feces; Transmission mode; Comparative study
• ISSN: 0098-7484; 1538-3598
• DOI: 10.1001/jama.282.23.2240

CONTEXT Helicobacter pylori commonly infects humans; however, its mode of transmission remains unknown. OBJECTIVE To determine how humans—the primary host for H pylori—shed the organism into the environment. DESIGN Controlled clinical experimental study conducted from February through December 1998. SETTING Clinical research unit of a hospital in northern California. PATIENTS Sixteen asymptomatic H pylori–infected and 10 uninfected adults. INTERVENTION A cathartic (sodium phosphate) and an emetic (ipecac) were given to all infected subjects and an emetic was given to 1 uninfected subject. MAIN OUTCOME MEASURE Confirmed H pylori isolates cultured from stool, air, or saliva before and after catharsis and emesis and from vomitus during emesis. Isolates were fingerprinted using repetitive extragenic palindromic (REP) polymerase chain reaction and species identity was confirmed by sequencing the 16s ribosomal RNA gene. RESULTS All vomitus samples from infected subjects grew H pylori, often in high quantities. Air sampled during vomiting grew H pylori from 6 (37.5%) of the 16 subjects. Saliva before and after emesis grew low quantities of H pylori in 3 (18.8%) and 9 (56.3%) subjects, respectively. No normal stools and only 22 (21.8%) of 101 induced stools grew the organism, although 7 (50.0%) of 14 subjects had at least 1 positive culture (2 stool culture samples were contaminated by fungus and were not included). Fingerprints of isolates within subjects were identical to one another but differed among subjects. No samples from uninfected subjects yielded H pylori. CONCLUSIONS Helicobacter pylori can be cultivated uniformly from vomitus and, occasionally, from saliva and cathartic stools. The organism is potentially transmissible during episodes of gastrointestinal tract illness, particularly with vomiting.