Production, Characterization, and Application of an Alginate Lyase, AMOR_PL7A, from Hot Vents in the Arctic Mid-Ocean Ridge

• Published in: Journal of agricultural and food chemistry Vol. 67; no. 10; pp. 2936 - 2945
• Main Authors: Vuoristo, Kiira S, Fredriksen, Lasse, Oftebro, Maren, Arntzen, Magnus Ø, Aarstad, Olav A, Stokke, Runar, Steen, Ida H, Hansen, Line Degn, Schüller, Reidar B, Aachmann, Finn L, Horn, Svein J, Eijsink, Vincent G. H
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 13.03.2019
• Subjects: Arctic Regions; Bacteria - classification; Bacteria - enzymology; Bacteria - genetics; Bacteria - isolation & purification; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biocatalysis; Enzyme Stability; Hydrogen-Ion Concentration; Hydrothermal Vents - chemistry; Hydrothermal Vents - microbiology; Kinetics; Metagenomics; Phaeophyceae - chemistry; Phylogeny; Polysaccharide-Lyases - chemistry; Polysaccharide-Lyases - genetics; Polysaccharide-Lyases - metabolism; Polysaccharides - chemistry; Seaweed - chemistry; Substrate Specificity; Temperature; Arctic Regions; salt tolerance; alginate lyase; brown seaweed; PolyM; Saccharina latissima; biorefining
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/acs.jafc.8b07190

Enzymatic depolymerization of seaweed polysaccharides is gaining interest for the production of functional oligosaccharides and fermentable sugars. We describe a thermostable alginate lyase belonging to Polysaccharide Lyase family 7 (PL7), which can be used to degrade brown seaweed, Saccharina latissima, at conditions also suitable for a commercial cellulase cocktail (Cellic CTec2). This enzyme, AMOR_PL7A, is a β-d-mannuronate specific (EC 4.2.2.3) endoacting alginate lyase, which degrades alginate and poly mannuronate within a broad range of pH, temperature and salinity. At 65 °C and pH 6.0, its Km and k cat values for sodium alginate are 0.51 ± 0.09 mg/mL and 7.8 ± 0.3 s–1 respectively. Degradation of seaweed with blends of Cellic CTec2 and AMOR_PL7A at 55 °C in seawater showed that the lyase efficiently reduces viscosity and increases glucose solublization. Thus, AMOR_PL7A may be useful in development of efficient protocols for enzymatic seaweed processing.