Understanding the Effects of Genotype, Growing Year, and Breeding on Tunisian Durum Wheat Allergenicity. 2. The Celiac Disease Case

• Published in: Journal of agricultural and food chemistry Vol. 65; no. 28; pp. 5837 - 5846
• Main Authors: Boukid, Fatma, Prandi, Barbara, Sforza, Stefano, Sayar, Rhouma, Seo, Yong Weon, Mejri, Mondher, Yacoubi, Ines
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 19.07.2017
• Subjects: Breeding; Celiac Disease - immunology; Genotype; Humans; Plant Proteins - analysis; Plant Proteins - genetics; Plant Proteins - immunology; Seasons; Time Factors; Triticum - chemistry; Triticum - genetics; Triticum - growth & development; Triticum - immunology; Tunisia; celiac disease; toxic; gluten; immunogenic; MANOVA; breeding
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/acs.jafc.7b02041

The aim of this study was to compare immunogenic and toxic gluten peptides related to celiac disease (CD). 100 accessions of genotypes selected during the 20th century in Tunisia were in vitro digested and then analyzed by UPLC/ESI-MS technique using an isotopically labeled internal standard. The first MANOVA confirmed a high variability in the content of immunogenic and toxic peptides reflecting high genetic diversity in the germplasm released during the past century in Tunisia, consistently with PCA and clustering analysis results. Our finding showed also important variability in CD epitopes due to growing season’s climate scenarios. Moreover, the second MANOVA revealed significant differences between abandoned and modern cultivars’ CD-related peptide amounts. Although we could not conclude that there was an augment of allergens in newly selected durum wheat lines compared to abandoned ones, we demonstrated that modern genotype peptides were less sensitive to climate variation, which is a useful indicator for wheat breeders.