Production of Aromatic Minimal Polyketides by the Daunorubicin Polyketide Synthase Genes Reveals the Incompatibility of the Heterologous DpsY and JadI Cyclases

• Published in: Journal of natural products (Washington, D.C.) Vol. 64; no. 8; pp. 1077 - 1080
• Main Authors: Wohlert, Sven-Eric, Wendt-Pienkowski, Evelyn, Bao, Wuli, Hutchinson, C. Richard
• Format: Journal Article
• Language: English
• Published: WASHINGTON American Chemical Society 01.08.2001; Amer Chemical Soc; American Society of Pharmacognosy
• Subjects: Acetyl Coenzyme A - metabolism; Alcohol Oxidoreductases - metabolism; Anthraquinones - chemistry; Anti-Bacterial Agents - biosynthesis; Anti-Bacterial Agents - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biological and medical sciences; Biotechnology; Chemistry, Medicinal; Chromatography, High Pressure Liquid; Cyclization; Daunorubicin - chemistry; Doxorubicin - chemistry; Fundamental and applied biological sciences. Psychology; Gene Expression; Genes, Bacterial; Genetic engineering; Genetic technics; Health. Pharmaceutical industry; Industrial applications and implications. Economical aspects; Intramolecular Transferases - metabolism; Isoquinolines - chemistry; Life Sciences & Biomedicine; Magnetic Resonance Spectroscopy; Methods. Procedures. Technologies; Models, Molecular; Modification of gene expression level; Molecular Structure; Multienzyme Complexes - genetics; Multienzyme Complexes - metabolism; Other active biomolecules; Pharmacology & Pharmacy; Plant Sciences; Plasmids; Production of active biomolecules; Pyrones - chemistry; Pyrones - metabolism; Science & Technology; Streptomyces - enzymology; Streptomyces - genetics; Streptomyces - physiology; CHOICE; ANTIBIOTICS; DOXA; CLONING; DOXORUBICIN BIOSYNTHESIS; PURIFICATION; STARTER UNIT; AKLANONIC ACID; STREPTOMYCES-PEUCETIUS; STEPS; Antineoplastic agent; Recombinant microorganism; Benzophenone derivatives; Biosynthesis; Oxygen heterocycle; Actinomycetales; Daunorubicin; Gene; Production; Bacteria; Aromatic compound; Oxygen nitrogen heterocycle; Gene cluster; Streptomycetaceae; Enzyme; Polycyclic compound; Lactone; Gene expression; Plasmid; Antibiotic; Polyketides; Ketophenols; Streptomyces lividans; Benzenic compound; Actinomycetes; Anthracyclins
• ISSN: 0163-3864; 1520-6025
• DOI: 10.1021/np010067f

Our investigations into whether the biosynthesis of a linearly fused ring system of an aromatic polyketide (jadomycin) could be modified to produce an angularly fused system (daunorubicin) and vice versa showed that introduction of the respective cyclases did not have the desired effect. Genes from the daunorubicin pathway produced a novel 21-carbon polyketide.