BioPEGylation of Polyhydroxybutyrate Promotes Nerve Cell Health and Migration

• Published in: Biomacromolecules Vol. 15; no. 1; pp. 339 - 349
• Main Authors: Chan, Rodman T. H, Russell, Robert A, Marçal, Helder, Lee, Terry H, Holden, Peter J, Foster, L. John R
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 13.01.2014
• Subjects: Animals; Applied sciences; Biological and medical sciences; Biological properties; Cell Movement - drug effects; Cell Movement - physiology; Cell Proliferation - drug effects; Cell Survival - drug effects; Cell Survival - physiology; Cells, Cultured; Cupriavidus necator; Exact sciences and technology; Hydroxybutyrates - chemistry; Hydroxybutyrates - pharmacology; Medical sciences; Neurons - drug effects; Neurons - physiology; Organic polymers; Physicochemistry of polymers; Polyethylene Glycols - chemistry; Polyethylene Glycols - pharmacology; Polymers - chemistry; Polymers - pharmacology; Properties and characterization; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments; X-Ray Diffraction; Biological properties; Cell proliferation; End group; Cytotoxicity; Ethylene oxide polymer; Wettability; Cell cycle; Surface properties; Hydroxyl group; Bacteria; Biocompatibility; Biomaterial; Framework; Modified material; Glial cell; Tissue engineering; Mechanical properties; Crystallinity; Experimental study; Gene expression; In vitro; Ralstonia eutropha; Microbial origin; Microroughness; Cell migration
• ISSN: 1525-7797; 1526-4602
• DOI: 10.1021/bm401572a

This study reports on the superior suitability of Polyhydroxybutyrate-polyethylene glycol hybrid polymers biosynthesised by Cupriavidus necator over PHB as biomaterials for tissue engineering. Incorporation of PEG106 (DEG) during PHB biosynthesis reduced crystallinity, molecular weight, and hydrophobicity while improving mechanical properties. In vitro olfactory ensheathing cell (OEC) proliferation was enhanced by cultivation on PHB-b-DEG films. Cultivation on PHB and PHB-b-DEG films showed no cytotoxic responses and cell viability and membrane integrity was sustained. PHB-b-DEG films promoted OECs entering into the DNA replication (S) phase and mitotic (G2-M) phase during the cell growth cycle and apoptosis was low. This study also confirmed an association between the level of neurite-outgrowth inhibitory protein (Nogo) and receptor pair Ig-like receptor B (PirB) expression and cell proliferation, both being down-regulated in cells grown on hybrid films when compared with PHB and asynchronous growth. Thus, DEG-terminated PHB-based biomaterials have great potential as biological scaffolds supporting nerve repair.