Protein-Triggered Instant Disassembly of Biomimetic Layer-by-Layer Films

Layer-by-Layer (LbL) coatings are promising tools for the biofunctionalization of biomaterials, as they allow stress-free immobilization of proteins. Here, we explore the possibility to immobilize phosvitin, a highly phosphorylated protein viewed as a model of bone phosphoproteins and, as such, a po...

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Published inLangmuir Vol. 27; no. 23; pp. 14370 - 14379
Main Authors Abdelkebir, Khalil, Gaudière, Fabien, Morin-Grognet, Sandrine, Coquerel, Gérard, Atmani, Hassan, Labat, Béatrice, Ladam, Guy
Format Journal Article
LanguageEnglish
Published Washington, DC American Chemical Society 06.12.2011
Subjects
Biocompatible Materials - chemistry
Bioengineering
Biological Interfaces: Biocolloids, Biomolecular and Biomimetic Materials
Biomaterials
Chemical Sciences
Chemistry
Chondroitin Sulfates - chemistry
Exact sciences and technology
General and physical chemistry
Life Sciences
Membranes, Artificial
or physical chemistry
Phosvitin - chemistry
Polylysine - chemistry
Polymers
Theoretical and
Drug
Polyanion
Film
Immobilization
Calcium phosphate
Potential
Sulfates
Protein
Polyelectrolyte
Lysine
Biomaterial
Models
Bone
Deposition
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Summary:Layer-by-Layer (LbL) coatings are promising tools for the biofunctionalization of biomaterials, as they allow stress-free immobilization of proteins. Here, we explore the possibility to immobilize phosvitin, a highly phosphorylated protein viewed as a model of bone phosphoproteins and, as such, a potential promotive agent of surface-directed biomineralization, into biomimetic LbL architectures. Two immobilization protocols are attempted, first, using phosvitin as the polyanionic component of phosvitin/poly-(l-lysine) films and, second, adsorbing it onto preformed chondroitin sulfate/poly-(l-lysine) films. Surprisingly, it is neither possible to embed phosvitin as the constitutive polyanion of the LbL architectures nor to adsorb it atop preformed films. Instead, phosvitin triggers instant massive film disassembly. This unexpected, incidentally detected behavior constitutes the first example of destructive interactions between LbL films and a third polyelectrolyte, a fortiori a protein, which might open a route toward new stimuli-responsive films for biosensing or drug delivery applications. Interestingly, additional preliminary results still indicate a promotive effect of phosvitin-containing remnant films on calcium phosphate deposition.
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ISSN:0743-7463
1520-5827
DOI:10.1021/la2033109