Electrophoretic Time-of-Flight Measurements of Single DNA Molecules with Two Stacked Nanopores

• Published in: Nano letters Vol. 11; no. 11; pp. 5002 - 5007
• Main Authors: Langecker, Martin, Pedone, Daniel, Simmel, Friedrich C, Rant, Ulrich
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 09.11.2011
• Subjects: Applied sciences; Biosensing Techniques - instrumentation; Condensed matter: structure, mechanical and thermal properties; Deoxyribonucleic acid; Devices; DNA - analysis; DNA - chemistry; Dynamic tests; Electronics; Electrophoresis - instrumentation; Equipment Design; Equipment Failure Analysis; Exact sciences and technology; Gain; General equipment and techniques; Instruments, apparatus, components and techniques common to several branches of physics and astronomy; Low-dimensional structures (superlattices, quantum well structures, multilayers): structure, and nonelectronic properties; Molecular electronics, nanoelectronics; Nanostructure; Nanostructures - chemistry; Nanostructures - ultrastructure; Nanotechnology - instrumentation; Physics; Polymers; Porosity; Semiconductor electronics. Microelectronics. Optoelectronics. Solid state devices; Sensors (chemical, optical, electrical, movement, gas, etc.); remote sensing; Statistical analysis; Surfaces and interfaces; thin films and whiskers (structure and nonelectronic properties); Transport; mobility; zeta potential; translocation; Nanopore; DNA; time-of-flight; Translocation; Time dependence; Statistical analysis; Sensors; Polymers; Potassium chloride; Nanoporosity; Nanoelectronics
• ISSN: 1530-6984; 1530-6992
• DOI: 10.1021/nl2030079

Electrophoretic transport through a solid-state nanodevice comprised of two stacked nanopore sensors is used to determine the free-solution mobility of DNA molecules based on their “time-of-flight” between the two pores. Mobility measurements are possible at very low (100 pM) DNA concentration and for low as well as high salt concentrations (here 30 mM and 1 M KCl). The mechanism of DNA transport through the device is elucidated by statistical analysis, showing the free-draining nature of the translocating DNA polymers and a barrier-dominated escape through the second pore. Furthermore, consecutive threading of single molecules through the two pores can be used to gain more detailed information on the dynamics of the molecules by correlation analysis, which also provides a direct electrical proof for translocation.