Novel Natural Products from Soil DNA Libraries in a Streptomycete Host

As a route to accessing the potential chemical diversity of uncultivable microbes from the soil, combinatorial biosynthetic libraries were constructed by cloning large fragments of DNA isolated from soil into a Streptomyces lividans host. Four novel compounds, terragines A (1), B (2), C (3), and D (...

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Bibliographic Details
Published inOrganic letters Vol. 2; no. 16; pp. 2401 - 2404
Main Authors Wang, Gui-Yang-Sheng, Graziani, Edmund, Waters, Barbara, Pan, Wubin, Li, Xiang, McDermott, Joe, Meurer, Guido, Saxena, Geeta, Andersen, Raymond J, Davies, Julian
Format Journal Article
LanguageEnglish
Published WASHINGTON American Chemical Society 10.08.2000
Amer Chemical Soc
Subjects
Amides - chemistry
Amides - metabolism
Biological Products - chemistry
Biological Products - genetics
Chemistry
Chemistry, Organic
Cloning, Molecular
DNA, Bacterial - genetics
Gene Library
Molecular Structure
Physical Sciences
Science & Technology
Soil Microbiology
Streptomyces - genetics
Streptomyces - metabolism
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Summary:As a route to accessing the potential chemical diversity of uncultivable microbes from the soil, combinatorial biosynthetic libraries were constructed by cloning large fragments of DNA isolated from soil into a Streptomyces lividans host. Four novel compounds, terragines A (1), B (2), C (3), and D (4), were isolated from recombinant 436-s4-5b1, and another novel compound, terragine E (5), was isolated from 446-s3-102g1. The structures were determined by a combination of spectroscopic techniques, primarily 2D NMR.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:1523-7060
1523-7052
DOI:10.1021/ol005860z