Rapid, Noninvasive, and Nondestructive Method for Biofilm Imaging on Metallic Surfaces Using Active Thermography

A simple, rapid, low-cost method was proposed for the imaging of Pseudomonas aeruginosa biofilms on metallic surfaces using an infrared camera. Stainless steel coupons were cooled to generate a thermal gradient in relation to biofilm for active thermography (AT). Both cooling and image acquisition t...

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Published inAnalytical chemistry (Washington) Vol. 92; no. 8; pp. 5682 - 5687
Main Authors Voss, Mônica, Alessio, Keiti Oliveira, Vianna Santos, Roberto Christ, de Souza, Márcia Ebling, Clerici, Dariane Jornada, Wagner, Roger, Cichoski, Alexandre José, Costa, Adilson Ben da, Helfer, Gilson Augusto, Machado, Guilherme Saldanha, Barbosa, Jorge Luis Victoria, Müller, Edson Irineu, Barin, Juliano Smanioto
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 21.04.2020
Subjects
Anti-Bacterial Agents - pharmacology
Biofilms
Biofilms - drug effects
Chemistry
Computer programs
Food Microbiology
Image acquisition
Image processing
Infrared cameras
Low cost
Microbial Sensitivity Tests
Nondestructive testing
Peracetic acid
Peracetic Acid - pharmacology
Pseudomonas aeruginosa
Pseudomonas aeruginosa - drug effects
Scanning electron microscopy
Software
Stainless steel
Stainless Steel - chemistry
Stainless steels
Surface Properties
Thermography
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Summary:A simple, rapid, low-cost method was proposed for the imaging of Pseudomonas aeruginosa biofilms on metallic surfaces using an infrared camera. Stainless steel coupons were cooled to generate a thermal gradient in relation to biofilm for active thermography (AT). Both cooling and image acquisition times were optimized and the images obtained with AT were compared with those from scanning electron microscopy. A free software (Thermofilm) was developed for image processing and the results were compared with the software ImageJ, with good agreement (from 87.7 to 103.8%). Images of coupons treated with sanitizer (peracetic acid) were obtained to show the applicability of the proposed method for biofilm studies. All analytical steps could be performed in 3 min in a noncontact, nondestructive, low-cost, portable, and easy-to-use way.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.9b05713