Aptamer-Based Analysis and Manipulation of the Protein Activity in Living Cells

Directly analyzing and precisely manipulating the activity of target proteins without altering their natural structure and expression would be essential to decoding many protein-dominant cellular processes. To meet this goal, we used streptavidin as the carrier to develop an aptamer-based nanoplatfo...

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Bibliographic Details
Published inAnalytical chemistry (Washington) Vol. 94; no. 10; pp. 4352 - 4358
Main Authors Xun, Kanyu, Sun, Yue, Zhang, Qiang, Wen, Nachuan, Wang, Zhimin, Qiu, Liping, Tan, Weihong
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 15.03.2022
Subjects
Active control
Analytical chemistry
Aptamers
Aptamers, Nucleotide - chemistry
Cells (biology)
Cellular structure
Chemistry
Cytoplasm
Cytoplasm - metabolism
Nuclear transport
Oligonucleotides - metabolism
Protein Transport
Proteins
RelA protein
Signal Transduction
Streptavidin
Streptavidin - chemistry
Translocation
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Summary:Directly analyzing and precisely manipulating the activity of target proteins without altering their natural structure and expression would be essential to decoding many protein-dominant cellular processes. To meet this goal, we used streptavidin as the carrier to develop an aptamer-based nanoplatform for monitoring the activation process of specific proteins in living cells. Our results showed that this nanoplatform could efficiently enter the cellular cytoplasm and specifically report the presence of RelA in the activated state. Meanwhile, with incorporation of a photoresponsive module, this aptamer-based nanoplatform was able to manipulate the nuclear translocation behavior of active RelA, enabling control over related downstream signaling events.
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ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.1c05104