Enhanced MALDI MS Sensitivity by Weak Base Additives and Glycerol Sample Coating

The concept of rationally designing MALDI matrices has been extended to the next “whole sample” level. These studies have revealed some unexpected and exploitable insights in improving MALDI sensitivity. It is shown that (i) additives which only provide additional laser energy absorption are best to...

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Bibliographic Details
Published inAnalytical chemistry (Washington) Vol. 86; no. 1; pp. 744 - 751
Main Authors Cramer, Rainer, Karas, Michael, Jaskolla, Thorsten W
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 07.01.2014
Subjects
Additives
Amino Acid Sequence
Analytical chemistry
Animals
Cattle
Coating
Crystal structure
Energy absorption
Glycerol
Glycerol - chemistry
Glycerols
Lasers
Mathematical analysis
Molecular Sequence Data
Serum Albumin, Bovine - analysis
Serum Albumin, Bovine - genetics
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
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Summary:The concept of rationally designing MALDI matrices has been extended to the next “whole sample” level. These studies have revealed some unexpected and exploitable insights in improving MALDI sensitivity. It is shown that (i) additives which only provide additional laser energy absorption are best to be avoided; (ii) the addition of proton donors in the form of protonated weak bases can be highly beneficial; (iii) the addition of glycerol for coating crystalline samples is highly recommended. Overall, analytical sensitivity has been significantly increased compared to the current “gold” standards in MALDI MS, and new insights into the mechanisms and processes of MALDI have been gained.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0003-2700
1520-6882
DOI:10.1021/ac403228d