Non-Invasive Plasmonic-Based Real-Time Characterization of Cardiac Drugs on Cardiomyocytes Functional Behavior

In the fabrication of cardiac tissue, an important factor is continuous measurement of its contraction features. A module that allows for a dynamic system capable of noninvasive and label-free monitoring of the contraction profile under administering chemicals and drugs is highly valuable for unders...

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Published inAnalytical chemistry (Washington) Vol. 92; no. 2; pp. 2244 - 2250
Main Authors Mozneb, Maedeh, Mirza, Asad M, Li, Chen-Zhong
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 21.01.2020
Subjects
Analytical chemistry
Animals
Calcium
Calcium signalling
Cardiomyocytes
Cells, Cultured
Cellular structure
Chemistry
Continuous flow
Contraction
Drug screening
Drugs
Fabrication
Flow velocity
Heart
Heterocyclic Compounds, 4 or More Rings - administration & dosage
Heterocyclic Compounds, 4 or More Rings - pharmacology
Muscle contraction
Myocytes, Cardiac - drug effects
Organic chemistry
Rats
Real time
Structure-function relationships
Surface Plasmon Resonance
Time Factors
Tissues
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Summary:In the fabrication of cardiac tissue, an important factor is continuous measurement of its contraction features. A module that allows for a dynamic system capable of noninvasive and label-free monitoring of the contraction profile under administering chemicals and drugs is highly valuable for understanding accurate tissue mechanobiology. In this research, we have successfully demonstrated the use of surface plasmon resonance (SPR) technology for the first time to characterize the contractility of cardiac cells in response to Blebbistatin and ATP drug exposure in real tme. An optimal flow rate of 10 μL/min was selected for a continuous flow of warm media,and 10 μM drug administration effect was detected with high spatiotemporal sensitivity on contracting cardiomyocytes. Our drug screening has identified the source of the SPR periodic signal to be direct cell contraction rather than action potentials or calcium signaling. Per our results, SPR has high potential in applications in least-interference real-time and label-free tissue characterizations and cellular properties analysis from a functional and structural point of view.
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ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.9b04956