In Silico Selection and Validation of High-Affinity ssDNA Aptamers Targeting Paromomycin

• Published in: Analytical chemistry (Washington) Vol. 95; no. 27; pp. 10405 - 10413
• Main Authors: Li, Jiaqing, Liu, Yangyang, Liu, Dongdong, Xu, Tong, Zhang, Chen, Li, Jianjun, Wang, Zhuo A., Du, Yuguang
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 11.07.2023
• Subjects: Aptamers; Binding; Biosynthesis; Chemistry; Flexible structures; Genes; Glycan; Glycosylation; Paromomycin; Polysaccharides; rRNA; Screening; Workflow
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/acs.analchem.3c01575

Glycans are promising for disease diagnosis since glycan biosynthesis is significantly affected by disease states, and glycosylation changes are probably more pronounced than protein expression during the transformation to the diseased condition. Glycan-specific aptamers can be developed for challenging applications such as cancer targeting; however, the high flexibility of glycosidic bonds and scarcity of studies on glycan–aptamer binding mechanisms increased the difficulty of screening. In this work, the model of interactions between glycans and ssDNA aptamers synthesized based on the sequence of rRNA genes was developed. Our simulation-based approach revealed that paromomycin as a representative example of glycans is preferred to bind base-restricted stem structures of aptamers because they are more critical in stabilizing the flexible structures of glycans. Combined experiments and simulations have identified two optimal mutant aptamers. Our work would provide a potential strategy that the glycan-binding rRNA genes could act as the initial aptamer pools to accelerate aptamer screening. In addition, this in silico workflow would be potentially applied in the more extensive in vitro development and application of RNA-templated ssDNA aptamers targeting glycans.