Functionalization of Magnetic Nanowires by Charged Biopolymers

• Published in: Biomacromolecules Vol. 9; no. 9; pp. 2517 - 2522
• Main Authors: Magnin, D, Callegari, V, Mátéfi-Tempfli, S, Mátéfi-Tempfli, M, Glinel, K, Jonas, A. M, Demoustier-Champagne, S
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.09.2008
• Subjects: Applied sciences; Biological and medical sciences; Biopolymers - chemistry; Biotechnology; Carbohydrate Conformation; Carbohydrate Sequence; Chitosan - chemistry; Coated Materials, Biocompatible - chemistry; Composites; Exact sciences and technology; Forms of application and semi-finished materials; Fundamental and applied biological sciences. Psychology; Glucans - chemistry; Immobilization of enzymes and other molecules; Immobilization techniques; Magnetics; Materials Testing; Methods. Procedures. Technologies; Molecular Sequence Data; Nanowires - chemistry; Nickel - chemistry; Particle Size; Polymer industry, paints, wood; Surface Properties; Technology of polymers; Ultrasonics; Self-assembled layer; Self assembly; Glucose oxidase; Support; Enzyme; Immobilization; Multiple layer; Pullulan derivative; Metal; Experimental study; Polyelectrolyte; Magnetic material; Oside polymer; Polyacid; Nanocomposite; Nickel; Oxidoreductases; Chitosan; Manufacturing; Nanowires; Immobilized enzyme
• ISSN: 1525-7797; 1526-4602
• DOI: 10.1021/bm8005402

We report on a facile method for the preparation of biocompatible and bioactive magnetic nanowires. The method consists of the direct deposition of polysaccharides by layer-by-layer (LbL) assembly onto a brush of metallic nanowires obtained by electrodeposition of the metal within the nanopores of an alumina template supported on a silicon wafer. Carboxymethylpullulan (CMP) and chitosan (CHI) multilayers were grown on brushes of Ni nanowires; subsequent grafting of an enzyme was performed by conjugating free amine side groups of chitosan with carboxylic groups of the enzyme. The nanowires are finally released by a gentle ultrasonic treatment. Transmission electron microscopy, electron energy-dispersive loss spectroscopy, and x-ray photoelectron spectroscopy indicate the formation of an homogeneous coating onto the nickel nanowires when one, two, or three CMP/CHI bilayers are deposited. This easy and efficient route to the biochemical functionalization of magnetic nanowires could find widespread use for the preparation of a broad range of nanowires with tailored surface properties.