Technique of Surface Modification of a Cell-Adhesion-Resistant Hydrogel by a Cell-Adhesion-Available Inorganic Microarray

• Published in: Biomacromolecules Vol. 9; no. 10; pp. 2569 - 2572
• Main Authors: Sun, Jianguo, Graeter, Stefan V, Yu, Lin, Duan, Shifeng, Spatz, Joachim P, Ding, Jiandong
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.10.2008
• Subjects: Acrylates - chemistry; Applied sciences; Biocompatible Materials - chemistry; Cell Adhesion; Exact sciences and technology; Glass; Gold - chemistry; Hydrogels - chemistry; Inorganic Chemicals - chemistry; Materials Testing; Microscopy, Electron, Scanning - methods; Oligonucleotide Array Sequence Analysis; Organic polymers; Physicochemistry of polymers; Polyethylene Glycols - chemistry; Polymers - chemistry; Properties and characterization; Protein Array Analysis; Solution and gel properties; Sulfhydryl Compounds - chemistry; Biological properties; Immersion method; Gold; Patterning; 3T3-Cell line; Metal; Experimental study; Ethylene oxide polymer; Array; Acrylate polymer; Cell adhesion; Biocompatibility; Hydrogel; Fibroblast
• ISSN: 1525-7797; 1526-4602
• DOI: 10.1021/bm800477s

A microtransfer technique for micropattern fabrication using a dithiol macromolecular linker is suggested by transferring a conventionally photolithography-prepared gold microarray on a hard inorganic substrate to a polymeric substrate. The linker was synthesized by end-capping a poly(ethylene glycol) (PEG) chain by the thiol groups. The efficiency of this technique is demonstrated by the transfer of gold microdots from glass to a cell-adhesion-resistant PEG hydrogel, which was formed by polymerizing PEG diacrylate macromers. The stability and biocompatibility of the resulting polymeric-inorganic hybrid material and cell-adhesion contrast of the patterned surface is confirmed by preliminary cell experiments.