Simple Approach to Stabilized Micelles Employing Miktoarm Terpolymers and Stereocomplexes with Application in Paclitaxel Delivery

• Published in: Biomacromolecules Vol. 10; no. 6; pp. 1460 - 1468
• Main Authors: Nederberg, Fredrik, Appel, Eric, Tan, Jeremy P. K, Kim, Sung Ho, Fukushima, Kazuki, Sly, Joseph, Miller, Robert D, Waymouth, Robert M, Yang, Yi Yan, Hedrick, James L
• Format: Journal Article
• Language: English
• Published: WASHINGTON American Chemical Society 08.06.2009; Amer Chemical Soc
• Subjects: Applied sciences; Biochemistry & Molecular Biology; Biological and medical sciences; Calorimetry, Differential Scanning; Chemistry; Chemistry, Organic; Exact sciences and technology; General pharmacology; Life Sciences & Biomedicine; Magnetic Resonance Spectroscopy; Medical sciences; Micelles; Microscopy, Electron, Transmission; Organic polymers; Paclitaxel - administration & dosage; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Physical Sciences; Physicochemistry of polymers; Polymer Science; Polymers - chemistry; Polymers with particular properties; Preparation, kinetics, thermodynamics, mechanism and catalysts; Science & Technology; Spectrometry, Fluorescence; THIN-FILMS; IN-VITRO RELEASE; NANOPARTICLES; DRUG-DELIVERY; CYCLIC CARBONATE; LIVING POLYMERIZATION; RING-OPENING POLYMERIZATION; BLOCK-COPOLYMER MICELLES; TRANSFER RADICAL POLYMERIZATION; ANTITUMOR-ACTIVITY; Ring opening polymerization; Terpolymer; Nitroxyl; Control release polymer; Lactic acid copolymer; Drug carrier; Stereocomplex; Ethylene oxide copolymer; Experimental study; Dimension spectrum; In vitro; Star copolymer; Y shape; Monodispersed polymer; Optically active copolymer; Free radical polymerization; Preparation; Paclitaxel; Kinetics; Aqueous solution; Micellar solution; Block copolymer; Release; Amphiphilic polymer
• ISSN: 1525-7797; 1526-4602
• DOI: 10.1021/bm900056g

A simple and versatile approach to miktoarm co- and terpolymers from carbonate functional oligomers is described. The key building block employed is a carboxylic acid functional cyclic carbonate, derived from 2,2-bis(methylol)propionic acid, that was readily coupled to a hydroxyl functional monomethylether poly(ethylene glycol) oligomer. Ring-opening of the cyclic carbonate using functional amines generates a carbamate linkage bearing a functional group capable of initiating either controlled radical or ring-opening polymerization, together with a primary hydroxyl group for ring-opening polymerization. Two tandem polymerization steps were possible which add the second two arms, thus generating the targeted ABC miktoarm terpolymer. The resulting amphiphilic miktoarm terpolymers containing poly(d- and l-lactide) formed polylactide stereocomplexes in the bulk. In aqueous solution, the stereocomplex mixture of Y-shaped miktoarm copolymers, poly(ethylene glycol)-poly(d-lactide)-poly(d-lactide) and poly(ethylene glycol)-poly(l-lactide)-poly(l-lactide), or the stereoblock miktoarm poly(ethylene glycol)-poly(d-lactide)-poly(l-lactide) form stabilized micelles with a significantly lower critical micelle concentration than those derived from conventional stereo regular linear or Y-shaped amphiphiles. This simple and versatile approach provides a useful synthetic route to complex macromolecular architectures that can assemble into stable micelles. These micelles provide high capacity for loading of the anticancer drug paclitaxel and possess narrow size distribution as well as unique structure, leading to sustained and near zero-ordered release of drug without significant initial burst.