Expression Of Wild-Type and Mutant Murine .alpha.-Lactalbumin cDNAs in Baculovirus-Infected Insect Cells

• Published in: Journal of agricultural and food chemistry Vol. 43; no. 5; pp. 1392 - 1395
• Main Authors: Soulier, Solange, Vilotte, Jean-luc, Mercier, Jean-Claude
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.05.1995
• Subjects: ADN; ALBUMINAS; ALBUMINE; baculovirus; Biological and medical sciences; Biotechnology; CULTIVO DE CELULAS; CULTURE DE CELLULE; EXPRESION GENICA; EXPRESSION DES GENES; Food engineering; Fundamental and applied biological sciences. Psychology; Genetic engineering; Genetic technics; GLICOSILTRANSFERASAS; GLYCOSYLTRANSFERASE; LACTOSA; LACTOSE; Life Sciences; METABOLISME DES GLUCIDES; METABOLISMO DE CARBOHIDRATOS; Methods. Procedures. Technologies; MUTACION; MUTANT; MUTANTES; MUTATION; PROTEINAS; PROTEINE; RATON; SOURIS; SPODOPTERA FRUGIPERDA; Vectors (cloning, transfer, expression). Insertion sequences and transposons; VIRUS POLIEDROSIS NUCLEAR; VIRUS POLYEDROSE NUCLEAIRE; Insecta; Site directed mutagenesis; Spodoptera frugiperda; Baculovirus; Gene expression; Virus; Cell line; Baculoviridae; Lactalbumin; Arthropoda; Lepidoptera; Noctuidae; Recombinant protein; Invertebrata; Vector; Comparative study; ADN C
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf00053a048

Production of wild-type and mutant mouse alpha-lactalbumins in baculovirus-infected insect cells is described. The efficiency of this expression system and its adequacy for studying the structure-function relationship in this subunit of lactose synthase are compared to those previously reported. Enzymatic characterization of the recombinant proteins is reported. The wild-type protein behaves as milk-purified alpha-lactalbumin, and one of the mutants was found to be unable to induce lactose synthesis in vitro