Survival of Adenovirus Types 2 and 41 in Surface and Ground Waters Measured by a Plaque Assay

• Published in: Environmental science & technology Vol. 45; no. 9; pp. 4145 - 4150
• Main Authors: Rigotto, C, Hanley, K, Rochelle, P.A, De Leon, R, Barardi, C.R.M, Yates, M.V
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01.05.2011
• Subjects: Adenoviridae - physiology; Adenoviridae Infections - virology; Adenoviruses; Animal, plant and microbial ecology; Applied ecology; Aquifers; Bioassays; Biological and medical sciences; Cell Line, Tumor; Ecotoxicology and Human Environmental Health; Ecotoxicology, biological effects of pollution; Fresh Water - virology; Fundamental and applied biological sciences. Psychology; General aspects; Groundwater; Humans; Kinetics; Survival analysis; Temperature; Viral Plaque Assay; Water Microbiology; Water Supply; Water treatment; Assay; Survival; Surface water; Ground water
• ISSN: 0013-936X; 1520-5851
• DOI: 10.1021/es103922r

To manage artificial recharge systems, it is necessary to understand the inactivation process of microorganisms within aquifers so that requirements regarding storage times and treatment strategies for ground and surface waters can be developed and modeled to improve water management practices. This study was designed to investigate the survival of representative adenoviruses in surface- and groundwaters using a cell culture plaque assay with human lung carcinoma cells (A549) to enumerate surviving viruses. Adenovirus types 2 (Ad2) and 41 (Ad41) were seeded into 50 mL of three sterilized surface waters and groundwaters, and incubated at 10 and 19 °C for up to 301 days. Concentrations of Ad2 and Ad41 were relatively stable in all waters at 10 °C for at least 160 days and in some instances up to 301 days. At 19 °C, virus concentrations were reduced by 99.99% (4 log) after 301 days in surface water. There was approximately 90% (1 log) reduction of both viruses at 19 °C after 160 days of incubation in groundwater samples. There was no overall difference in survival kinetics in surface waters compared to groundwaters. The relatively high stability and long-term survival of adenoviruses in environmental waters at elevated temperatures should be considered in risk assessment models and drinking water management strategies.