Secretome of the Biocontrol Agent Metarhizium anisopliae Induced by the Cuticle of the Cotton Pest Dysdercus peruvianus Reveals New Insights into Infection

Metarhizium anisopliae is an entomopathogenic fungus that has evolved specialized strategies to infect insect hosts. Here we analyzed secreted proteins related to Dysdercus peruvianus infection. Using shotgun proteomics, abundance changes in 71 proteins were identified after exposure to host cuticle...

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Published inJournal of proteome research Vol. 13; no. 5; pp. 2282 - 2296
Main Authors Beys-da-Silva, Walter O, Santi, Lucélia, Berger, Markus, Calzolari, Diego, Passos, Dario O, Guimarães, Jorge A, Moresco, James J, Yates, John R
Format Journal Article
LanguageEnglish
Published United States American Chemical Society 02.05.2014
Subjects
Animals
Cell Surface Extensions - microbiology
Fungal Proteins - metabolism
Fungal Proteins - secretion
Gossypium - parasitology
Heteroptera - microbiology
Host-Pathogen Interactions
Metarhizium - metabolism
Metarhizium - physiology
Proteome - metabolism
Proteome - secretion
Proteomics - methods
Tandem Mass Spectrometry
host infection
Metarhizium anisopliae
biocontrol
Dysdercus peruvianus
shotgun proteomics
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Summary:Metarhizium anisopliae is an entomopathogenic fungus that has evolved specialized strategies to infect insect hosts. Here we analyzed secreted proteins related to Dysdercus peruvianus infection. Using shotgun proteomics, abundance changes in 71 proteins were identified after exposure to host cuticle. Among these proteins were classical fungal effectors secreted by pathogens to degrade physical barriers and alter host physiology. These include lipolytic enzymes, Pr1A, B, C, I, and J proteases, ROS-related proteins, oxidorreductases, and signaling proteins. Protein interaction networks were generated postulating interesting candidates for further studies, including Pr1C, based on possible functional interactions. On the basis of these results, we propose that M. anisopliae is degrading host components and actively secreting proteins to manage the physiology of the host. Interestingly, the secretion of these factors occurs in the absence of a host response. The findings presented here are an important step in understanding the host–pathogen interaction and developing more efficient biocontrol of D. peruvianus by M. anisopliae.
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ISSN:1535-3893
1535-3907
DOI:10.1021/pr401204y