Influence of Temperature and Humidity on the Stability of Carotenoids in Biofortified Maize (Zea mays L.) Genotypes during Controlled Postharvest Storage

• Published in: Journal of agricultural and food chemistry Vol. 64; no. 13; pp. 2727 - 2736
• Main Authors: Ortiz, Darwin, Rocheford, Torbert, Ferruzzi, Mario G
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 06.04.2016
• Subjects: Algorithms; biofortification; carotenoids; Carotenoids - analysis; Carotenoids - chemistry; corn; developmental stages; drying; Edible Grain - chemistry; Genotype; harvest date; Humidity; Models, Chemical; pollination; porosity; postharvest treatment; relative humidity; seed development; seeds; storage time; surface area; Temperature; water content; Zea mays; Zea mays - chemistry; Zea mays - genetics; carotenoid degradation; biofortified corn; nutritional impact; retention; postharvest storage
• ISSN: 0021-8561; 1520-5118; 1520-5118
• DOI: 10.1021/acs.jafc.5b05698

Maize is a staple crop that has been the subject of biofortification efforts to increase the natural content of provitamin A carotenoids. Although significant progress toward increasing provitamin A carotenoid content in maize varieties has been made, postharvest handling factors that influence carotenoid stability during storage have not been fully established. The objectives of this study were to determine carotenoid profiles of six selected provitamin A biofortified maize genotypes at various developmental stages and assess the stability of carotenoids in maize kernels during controlled storage conditions (12 month period), including elevated temperature and relative humidity. There were no significant changes in the content of individual carotenoids within genotypes during kernel development from 45 days after pollination through the time of harvest. Carotenoid losses through traditional grain drying were also minimal (<9%). However, the stability of carotenoids in maize kernels over storage time after harvest was found to be dependent on both temperature and humidity, with variation observed among genotypes. Different forms of provitamin A carotenoids follow similar degradation rates. The genotype C17xDE3 had a degradation rate 2 times faster than those of the other genotypes evaluated (P < 0.001). These differences in carotenoid stability under controlled storage were attributed, in part, to observed differences in the physical properties of the kernels (surface area and porosity). These results support the notion that effective control of moisture content and temperature of the kernels during storage conditions is essential to reduce the speed of degradative reactions.