Transepithelial Transports of Rare Sugar d‑Psicose in Human Intestine

• Published in: Journal of agricultural and food chemistry Vol. 61; no. 30; pp. 7381 - 7386
• Main Authors: Hishiike, Takashi, Ogawa, Masahiro, Hayakawa, Shigeru, Nakajima, Daichi, O’Charoen, Siwaporn, Ooshima, Hisaka, Sun, Yuanxia
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 31.07.2013
• Subjects: Biological and medical sciences; Biological Transport; Caco-2 Cells; enterocytes; Enterocytes - chemistry; Enterocytes - metabolism; Food industries; forskolin; fructose; Fructose - chemistry; Fructose - metabolism; Fundamental and applied biological sciences. Psychology; Glucose - metabolism; Glucose Transporter Type 2 - metabolism; Glucose Transporter Type 5 - metabolism; Humans; Intestines - chemistry; Intestines - metabolism; Kinetics; Permeability; psicose; GLUT2; rare sugar; Caco-2; GLUT5; Human; Digestive system; Intestine; Biological transport; Gut; Sugar
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf401449m

d-Psicose (Psi), the C3-epimer of d-fructose (Fru), is a noncalorie sugar with a lower glycemic response. The trans-cellular pathway of Psi in human enterocytes was investigated using a Caco-2 cell monolayer. The permeation rate of Psi across the monolayer was not affected by the addition of phlorizin, an inhibitor of sugar transporter SGLT1, whereas it was accelerated by treatment with forskolin, a GLUT5-gene inducer, clearly showing that GLUT5 is involved in the transport of Psi. The permeability of Psi was suppressed in the presence of d-glucose (Glc) and Fru, suggesting that the three monosaccharides are transported via the same transporter. Since GLUT2, the predominant sugar transporter on the basolateral membrane of enterocytes, mediates the transport of Glc and Fru, Psi might be mediated by GLUT2. The present study shows that Psi is incorporated from the intestinal lumen into enterocytes via GLUT5 and is released to the lamina propria via GLUT2.