Purification and Characterization of Peptides Inhibiting MMP‑1 Activity with C Terminate of Gly-Leu from Simulated Gastrointestinal Digestion Hydrolysates of Tilapia (Oreochromis niloticus) Skin Gelatin

• Published in: Journal of agricultural and food chemistry Vol. 66; no. 3; pp. 593 - 601
• Main Authors: Liping, Sun, Qiuming, Liu, Jian, Fan, Xiao, Li, Yongliang, Zhuang
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 24.01.2018
• Subjects: collagen; digestion; fibroblasts; gastrointestinal system; gel chromatography; gelatin; hydrogen bonding; hydrolysates; interstitial collagenase; mice; Oreochromis niloticus; peptides; photoaging; protective effect; reactive oxygen species; reversed-phase high performance liquid chromatography; ultraviolet radiation; collagen type I; oxidant stress; molecular docking; UV radiation B; matrix metalloproteinases
• ISSN: 0021-8561; 1520-5118; 1520-5118
• DOI: 10.1021/acs.jafc.7b04196

Tilapia skin gelatin hydrolysates (TSGHs) were prepared by simulated gastrointestinal digestion and separated by gel filtration and semi-preparative reversed-phase high-performance liquid chromatography. The anti-photoaging effects were evaluated using an ultraviolet radiation B (UVB)-induced mouse embryonic fibroblast (MEF) photoaging model in vitro. Three fractions from TSGHs with high inhibitory intercellular matrix metalloproteinase-1 (MMP-1) activities and reactive oxygen species (ROS) production were obtained. Three key peptides, GYTGL, LGATGL, and VLGL, were identified, and their C terminate was Gly-Leu. Three peptides were synthesized and exhibited a significant inhibition of intercellular MMP-1 activity and ROS production. Furthermore, three peptides inhibiting MMP-1 activities were evaluated through their docking of S1′ and S3′ active pockets of MMP-1. Hydrogen bonds and C terminate Gly-Leu played important roles. Finally, the protective effects of three peptides on intercellular collagen in UVB-induced MEFs were compared. Our results indicated that tilapia gelatin peptides exhibited potential activities to prevent and regulate photoaging.