Expression of PAT and NPT II Proteins during the Developmental Stages of a Genetically Modified Pepper Developed in Korea

• Published in: Journal of agricultural and food chemistry Vol. 58; no. 20; pp. 10906 - 10910
• Main Authors: Kim, Hyo Jin, Lee, Si Myung, Kim, Jae Kwang, Ryu, Tae Hun, Suh, Seok Cheol, Cho, Hyun Suk
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 27.10.2010
• Subjects: Acetyltransferases - genetics; Acetyltransferases - metabolism; Biological and medical sciences; Capsicum; Capsicum - enzymology; Capsicum - genetics; Capsicum - growth & development; Chemical Aspects of Biotechnology/Molecular Biology; developmental stages; enzyme-linked immunosorbent assay; flowering; Food industries; Fruit and vegetable industries; fruits (plant anatomy); Fundamental and applied biological sciences. Psychology; gene expression; Gene Expression Regulation, Developmental; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; General aspects; hot peppers; Hygiene and safety; kanamycin kinase; Kanamycin Kinase - genetics; Kanamycin Kinase - metabolism; leaves; neomycin phosphotransferase II; phosphinothricin acetyltransferase; plant development; Plants, Genetically Modified - enzymology; Plants, Genetically Modified - genetics; Plants, Genetically Modified - growth & development; pollen; protein synthesis; Republic of Korea; roots; transgenic plants; South Korea; Asia; Korea; Republic of Korea; genetically modified pepper; neomycin phosphotransferase II; Safety assessment; phosphinothricin acetyltransferase; enzyme-linked immunosorbent assay; Vegetables; Pepper; Bell pepper; Enzyme immunoassay; Protein; Assay; Antibiotic; Neomycin; Developmental stage; Safety; Genetically modified organism; ELISA assay

Estimation of the protein levels introduced in a biotechnology-derived product is conducted as part of an overall safety assessment. An enzyme-linked immunosorbent assay (ELISA) was used to analyze phosphinothricin acetyltransferase (PAT) and neomycin phosphotransferase II (NPT II) protein expressio...