Expression of PAT and NPT II Proteins during the Developmental Stages of a Genetically Modified Pepper Developed in Korea

• Published in: Journal of agricultural and food chemistry Vol. 58; no. 20; pp. 10906 - 10910
• Main Authors: Kim, Hyo Jin, Lee, Si Myung, Kim, Jae Kwang, Ryu, Tae Hun, Suh, Seok Cheol, Cho, Hyun Suk
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 27.10.2010
• Subjects: Acetyltransferases - genetics; Acetyltransferases - metabolism; Biological and medical sciences; Capsicum; Capsicum - enzymology; Capsicum - genetics; Capsicum - growth & development; Chemical Aspects of Biotechnology/Molecular Biology; developmental stages; enzyme-linked immunosorbent assay; flowering; Food industries; Fruit and vegetable industries; fruits (plant anatomy); Fundamental and applied biological sciences. Psychology; gene expression; Gene Expression Regulation, Developmental; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; General aspects; hot peppers; Hygiene and safety; kanamycin kinase; Kanamycin Kinase - genetics; Kanamycin Kinase - metabolism; leaves; neomycin phosphotransferase II; phosphinothricin acetyltransferase; plant development; Plants, Genetically Modified - enzymology; Plants, Genetically Modified - genetics; Plants, Genetically Modified - growth & development; pollen; protein synthesis; Republic of Korea; roots; transgenic plants; South Korea; Asia; Korea; Republic of Korea; genetically modified pepper; neomycin phosphotransferase II; Safety assessment; phosphinothricin acetyltransferase; enzyme-linked immunosorbent assay; Vegetables; Pepper; Bell pepper; Enzyme immunoassay; Protein; Assay; Antibiotic; Neomycin; Developmental stage; Safety; Genetically modified organism; ELISA assay
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf1026907

Estimation of the protein levels introduced in a biotechnology-derived product is conducted as part of an overall safety assessment. An enzyme-linked immunosorbent assay (ELISA) was used to analyze phosphinothricin acetyltransferase (PAT) and neomycin phosphotransferase II (NPT II) protein expression in a genetically modified (GM) pepper plant developed in Korea. PAT and NPT II expression levels, based on both dry weight and fresh weight, were variable among different plant generations and plant sections from isolated genetically modified organism (GMO) fields at four developmental stages. PAT expression was highest in leaves at anthesis (11.44 μg/gdw and 2.17 μg/gfw) and lowest in roots (0.12 μg/gdw and 0.01 μg/gfw). NPT II expression was also highest in leaves at anthesis (17.31 μg/gdw and 3.41 μg/gfw) and lowest in red pepper (0.65 μg/gdw and 0.12 μg/gfw). In pollen, PAT expression was 0.59−0.62 μg/gdw, while NPT II was not detected. Both PAT and NPT II showed a general pattern of decreased expression with progression of the growing season. As expected, PAT and NPT II protein expression was not detectable in control pepper plants.