Effect of Protein on the Detection of Fluoroquinolone Residues in Fish Meat

• Published in: Journal of agricultural and food chemistry Vol. 60; no. 7; pp. 1722 - 1727
• Main Authors: Li, Huifang, Yin, Jungang, Liu, Yongming, Shang, Jian
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 22.02.2012
• Subjects: Acetonitriles; Animals; Anti-Bacterial Agents - analysis; Biological and medical sciences; Carps; Chromatography, High Pressure Liquid; fish; fish meat; Fish Proteins - metabolism; fluorescence; fluoroquinolones; Fluoroquinolones - analysis; Fluoroquinolones - chemistry; Fluoroquinolones - metabolism; Food industries; Fundamental and applied biological sciences. Psychology; General aspects; high performance liquid chromatography; Hydrogen Bonding; Meat - analysis; Meat and meat product industries; Methods of analysis, processing and quality control, regulation, standards; Muscles - chemistry; Reproducibility of Results; serum albumin; Serum Albumin - chemistry; Serum Albumin - metabolism; solid phase extraction; Spectrometry, Fluorescence; spectroscopy; liquid chromatography; fluoroquinolones; molecular fluorescence spectrometry; protein; fish; Analysis method; Residue; Meat product; Liquid chromatography; Control method; Detection; Protein; Fluorescence spectrometry; Meat
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf2034658

Using fish serum albumin (FSA) as the model protein, molecular fluorescence spectrometry and high-performance liquid chromatography (HPLC) were applied to study the effect of protein on the extraction of fluoroquinolone (FQ) residues in fish meat. There was a strong interaction between FQs and protein through hydrogen bonds, which could be broken as protein degenerated with 60–100% (v/v) acetonitrile acid solution, and FQs bound with protein were released in various degrees. On the basis of the results, a novel sample preparation procedure loosely based on QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) methodology was developed for the determination of FQ residues in fish muscle samples, using 90% (v/v) acetonitrile acid solution as the extractant, combined with a dispersive solid-phase extraction (DSPE) cleanup step. Mean recoveries of four FQs from spiked samples at a concentration range of 50–200 ng g–1 were 73.3–95.9% with relative standard deviations (RSD) lower than 10.7%.