Sustainable Strategy Based on Induced Precipitation for the Purification of Phycobiliproteins

• Published in: ACS sustainable chemistry & engineering Vol. 9; no. 10; pp. 3942 - 3954
• Main Authors: Martins, Margarida, Soares, Bruna P, Santos, João H. P. M, Bharmoria, Pankaj, Torres Acosta, Mario A, Dias, Ana C. R. V, Coutinho, João A. P, Ventura, Sónia P. M
• Format: Journal Article
• Language: English
• Published: American Chemical Society 15.03.2021
• Subjects: R-phycoerythrin; Gracilaria gracilis; purification; phycobiliproteins; induced precipitation
• ISSN: 2168-0485; 2168-0485
• DOI: 10.1021/acssuschemeng.0c09218

Phycobiliproteins are fluorescent proteins mainly produced by red macroalgae and cyanobacteria. These proteins, essential to the survival of these organisms, find application in many fields of interest, from medical, pharmaceutical, and cosmetic to food and textile industries. The biggest obstacle to their use is the lack of simple environmental and economical sustainable methodologies to obtain these proteins with high purity. In this work, a new purification process is proposed based on the induced precipitation of the target proteins followed by ultrafiltration. Purities of 89.5% of both phycobiliproteins and 87.3% of R-phycoerythrin were achieved using ammonium sulfate and poly­(acrylic acid) sodium salts as precipitation agents (followed by an ultrafiltration step), while maintaining high recovery yields and protein structure stability. Environmental analysis performed to evaluate the proposed process shows that the carbon footprint for the proposed process is much lower than that reported for alternative methodology, and the economic analysis reveals the cost-effective character associated to its high performance. This work is a step toward more sustainable and effective methodologies/processes with high industrial potential.