Effects of Antithrombogenic Agents on Microvenous Anastomoses in a Rat Model

• Published in: Archives of otolaryngology--head & neck surgery Vol. 137; no. 2; pp. 170 - 174
• Main Authors: Harsha, Wayne J, Kau, Ryan L, Kim, Namou, Hayden, Richard E
• Format: Journal Article
• Language: English
• Published: Chicago, IL American Medical Association 01.02.2011
• Subjects: Anastomosis, Surgical; Animals; Aspirin - pharmacology; Biological and medical sciences; Drug therapy; Femoral Vein - surgery; Ketorolac Tromethamine - pharmacology; Medical research; Medical sciences; Microcirculation; Models, Animal; Otolaryngology; Otorhinolaryngology. Stomatology; Platelet Aggregation Inhibitors - pharmacology; Random Allocation; Rats; Rodents; Thrombosis; Ticlopidine - analogs & derivatives; Ticlopidine - pharmacology; Time Factors; Vascular Patency - drug effects; Venous Thrombosis - prevention & control; Vertebrata; Mammalia; Rat; Animal; Rodentia
• ISSN: 0886-4470; 2168-6181; 1538-361X; 2168-619X
• DOI: 10.1001/archoto.2010.256

OBJECTIVE To compare venous thrombosis rates among animals treated with aspirin, clopidogrel bisulfate, and ketorolac tromethamine using an anastomotic “tuck” model. DESIGN Single-blind randomized animal study. SETTING An animal laboratory at a tertiary care academic referral center. SUBJECTS Forty-two retired Lewis breeder rats divided into 3 equal groups. INTERVENTIONS Before surgical intervention, 1 group received aspirin (10 mg/kg) through gavage; 1 group, clopidogrel bisulfate (5 mg/kg) through gavage; and the final group, ketorolac tromethamine (3 mg/kg) through intramuscular injection. Each rat was then anesthetized, and the femoral veins were prepared bilaterally. A 180° venotomy was made, and the vessels were anastomosed with the tuck model set-up for anastomotic failure. The vessels were checked for patency every 15 minutes for 2 hours after clamp removal. MAIN OUTCOME MEASURES The rate of venous thrombosis and the time to thrombosis. RESULTS In both the aspirin and clopidogrel groups, 2 of 28 vessels (7%) were thrombosed. Thrombosis occurred in 3 of 28 vessels (11%) in the ketorolac group (P = .86). All thromboses in the aspirin and clopidogrel groups took place at 7.5 minutes after clamp removal. In the ketorolac group, the mean time to thrombosis was 7.5 minutes (range, 0-22.5 minutes). There was no difference in time to thrombosis among the 3 groups (P = .86). CONCLUSION Using a microvenous tuck model set-up for anastomotic failure, we found no difference in the rate of thrombosis or the time to thrombosis in rats pretreated with aspirin, clopidogrel, or ketorolac.Arch Otolaryngol Head Neck Surg. 2011;137(2):170-174-->